高效液相色谱-串联质谱法检测毛发中的美托尼秦OA

Metonitazene has appeared on the illicit drug market,which is a new type of synthetic opioid with an analgesic effect stronger than morphine and a toxicity similar to traditional opioids.This study aimed to develop a rapid method for the detection of metonitazene in human hair by UPLC-MS/MS,to provide basic data on the addictive and harmful effects of metonitazene,and accumulate the evidence of metonitazene abuse.The hair samples were ground and extracted by methanol and then filtered by microporous membrane.The filtrate was analyzed on UPLC with an Agilent ZORBAX Eclipse Plus C18(100 mm × 2.1 mm× 1.8 μm)column,and the gradient elution was performed with aqueous containing 0.1％formic acid and acetonitrile solution containing 0.1％formic acid,at a flow rate of 0.4 mL/min.The positive ion scan mode with multiple reaction monitoring(MRM)was used for the MS detection.The results demonstrated that the extraction efficiency was the highest when methanol as extraction solvent,and the chromatographic peaks of metonitazene in the hair samples were good shape and symmetry.The precursor ion of metonitazene was m/z 383.2,and the fragment ions were m/z 100.0、120.9、72.0、44.0.The linear relationship was good in the range of 0.01、1.0 ng/mg,R2＞0.998.The limits of detection(LOD)were 0.005 ng/mg and the limits of quantification(LOQ)were 0.01 ng/mg.Besides,the recoveries were 97.3％、111.9％,the matrix effects were 101.5％、113.3％,and the relative standard deviations(RSD)of the intra-day and inter-day precision were both less than 10％.The method has the advantages of simple operation,rapid detection,high sensitivity,high recovery,and good reproducibility.The method is proved reliability on real samples,which can meet the requirements of metonitazene detection in forensic identification.