伊犁地区野生鸭茅根腐病调查、病原菌鉴定及其生物学特性OA

In order to identify the pathogen causing root rot on wild Dactylis glomerata L.in Xinjiang and determine its biological characteristics,disease surveys and sampling were conducted in the distribution areas of wild D.glomerata in Zhaosu,Tekes,and Xinyuan Counties of Ili,Xinjiang.The pathogen was iso-lated from the roots of diseased plants using tissue isolation and single-spore purification.Pathogen identi-fication was performed through pathogenicity tests,morphological observation,and multi-gene phylogenet-ic analysis based on ITS,TEF-α,and RPB2 sequences.Biological characteristics were determined using the mycelial growth rate method.Field surveys revealed that the natural incidence of root rot on wild D.glomerata ranged from 20%to 72%.The pathogen was identified as Fusarium acuminatum.The optimal conditions for mycelial growth were 20℃,pH 7,soluble starch as the carbon source,and urea as the nitrogen source.The fungus grew rapidly on potato-carrot agar(PCA),oat meal agar(OMA),potato-sucrose agar(PSA),and Czapek agar.Complete darkness favored colony expansion.