不同植物生长调节剂对琐琐葡萄茎段快繁的影响OA

This study aims to screen the optimal culture media and plant growth regulators(PGRs)for primary culture,proliferation culture and rooting culture of Vitis vinifera Suosuo,a medicinal grape culti-var endemic to Xinjiang,and to establish an efficient stem segment-based rapid propagation system for this germplasm.Single-node stem segments of Vitis vinifera Suosuo were used as explants.For primary cul-ture,the optimal basal medium for axillary bud germination was screened from five media types:MS,WPM,B5,Nitsch and ER.For proliferation culture,a quadratic regression orthogonal rotation combination design was applied to investigate the effects of different combinations of 6-benzyladenine(6-BA),indole-3-butyric acid(IBA)and gibberellic acid(GA3)on bud differentiation,proliferation and regeneration.For rooting culture,1/2-strength MS was used as the basal medium,with gradients of IBA and activated carbon(Ac)concentrations and different dark culture durations set to identify the optimal rooting formula and dark treatment conditions.The optimal medium for axillary bud germination of Vitis vinifera Suosuo stem segments was MS medium supplemented with 2.00 mg/L 6-BA,0.20 mg/L IBA,and 0.20 mg/L GA3,which achieved an axillary bud germination rate of 90.00%.The quadratic regression orthogonal rotationcom-bination analysis revealed that the order of significance of PGRs affecting stem segment proliferation was 6-BA>IBA>GA3,and the optimal proliferation formula was MS medium supplemented with 1.77 mg/L 6-BA,0.49 mg/L IBA,and 0.51 mg/L GA3.The best rooting performance was obtained with 1/2-strength MS medium supplemented with 1.50 mg/L IBA and 0.50 g/L Ac,combined with 5 days of dark culture,with a rooting rate reaching 88.89%.The optimized rapid propagation system for Vitis vinifera Suosuo stem segments is established as follows:MS medium is used for primary culture;the optimal PGR concentrations for proliferation culture are 1.77 mg/L 6-BA,0.49 mg/L IBA,and 0.51 mg/L GA3;the optimal conditions for rooting culture are 1.50 mg/L IBA,0.50 g/L Ac,combined with 5 days of dark cul-ture.