濒危植物平伐重楼(Paris vaniotii Lévl.)的繁育系统和传粉特性研究OA

To investigate the sexual reproduction characteristics of Paris vaniotii Lévl.during the flowering period and clarify their patterns,this study aims to enhance understanding of the reproduc-tive strategies of Paridis rhizoma and provide a theoretical basis for the conservation and artificial propagation of this endangered species.Floral morphology and structure were observed and recorded,and controlled pollination experiments,including bagging tests,were conducted to determine the pol-len-to-ovule ratio(P/O),outcrossing index(OCI),self-and cross-compatibility,and pollination mode(wind or insect).Flowering phenology,daily patterns of anther dehiscence,corresponding environ-mental conditions(temperature and humidity),and stigma secretion were also recorded.Stigma re-ceptivity was assessed using the benzidine-hydrogen peroxide method,while pollen viability was eval-uated using MTT staining and in vitro germination methods.The results showed that the P/O ratio was 5 860 and the OCI was 4,indicating that P.vaniotii was self-compatible but preferentially out-crossing.The flowering duration of a single flower lasted 17-19 days,and anther dehiscence occurred approximately four days after anthesis under favorable temperature(22.7℃-25.6℃),relative hu-midity(53.7%-46.1%),and adequate light conditions.The stigma remained receptive throughout the flowering period,and pollen retained viability during the entire anthesis.Stigma secreted mucus during the pollen-shedding stage,which likely facilitated pollination.Flowers of P.vaniotii are bisex-ual,self-compatible,and capable of both wind and insect pollination.Compared with P.polyphylla var.yunnanensis,the pollination window of P.vaniotii is narrower and more dependent on stable weather conditions,which may contribute to its endangered status.Ensuring stable environmental conditions during the flowering period is crucial for improving its sexual reproductive success.