白僵菌绿僵菌培养条件及生物防治应用探索OA

Beauveria bassiana and metarhizium anisopliae are important insect pathogenic fungi,and the optimization of culture conditions is crucial for biological control efficacy.Ultraviolet mutagenesis was employed to screen highly virulent strains,and solid-state fermentation process parameters were optimized through single-factor and response surface experiments.The results showed that the S2 mutant strain of Beauveria bassiana achieved a spore yield of 11.762×108 spores/g under a wheat bran-rice bran ratio of 1.34:1,representing a 3.05-fold increase compared to the original strain.Seed soaking treatment with metarhizium anisopliae significantly activated the maize defense enzyme system,with POD and SOD activities increasing by 1.7 and 1.4 fold,respectively.This study provides a technical foundation for the large-scale production and field application of insect pathogenic fungi.