"3414"施肥方案对'西豌1号'豌豆根际土壤微生物多样性和群落结构的影响OA

To explore the effects of different fertilization treatments on the structure and diversity of the rhizosphere soil microbial community of'Xiwan 1'pea,the"3414"fertilizer experiment design was adopted combined with high-throughput sequencing technology to analyze the soil microbial community structure and diversity under various fertilization regimes,as well as their correlations with soil physicochemical properties.The results showed that fertilization significantly increased the contents of total nitrogen(TN),available phosphorus(AP),available potassium(AK),and organic matter(SOM)content in soil,but had no significant effect on soil pH.Compared with N0P0K0,higher bacterial Alpha diversity was observed under the N2P0K2,N1P2K2,N2P1K2 and N2P2K2 treatments,while higher fungal Alpha diversity was found under the N2P2K1,N2P3K2,N2P1K1 and N1P2K2 treatments.The treatments of N2P1K1,N1P2K2,N2P2K3 and N2P1K2 significantly increased the relative abundances of dominant bacterial genera such as Sphingomicrobium,Streptomyces,Nocardioides and Blastococcus;the treatments of N2P3K2,N2P0K2,N2P2K0,N2P2K1 and N1P2K2 significantly increased the relative abundances of dominant fungal genera including Chae tomium,Pseudogymnoascus,Mortierella and Alternaria.The Spearman correlation heatmap analysis indicated that TN,AK and SOM were the main factors influencing of soil bacterial community,while AP and SOM were the primary factors affecting the fungal community.The study demonstrated that N1P2K2 treatment resulted in the highest richness and diversity of soil microorganisms,with higher Alpha diversity indices.Specifically,the relative abundance of Sphingomicrobium,Solirubrobacter and Alternaria,which are involved in organic matter degradation,Skermanella,which has nitrogen fixation function,and Mortierella,which has phosphorus-solubilizing function,were relatively increased.It significantly improved the content of TN,AP and SOM in the rhizosphere soil of peas.Therefore,rational fertilization could optimize soil nutrient content and regulate microbial community structure to provide an appropriate growth environment for plants.