TET1过表达对人肝癌HepG2细胞增殖和侵袭及裸鼠移植瘤生长的影响实验研究OA

Objective:To investigate the effects of Ten-eleven translocation methylcytosine di-oxygenase 1(TET1)overexpression on the proliferation and invasion of human hepatocellular carcinoma HepG2 cells and the growth of transplanted tumor in nude mice.Methods:Human hepatocellular carcinoma HepG2 cells were selected and divided into a blank group(without treatment),a negative control group(transfected with the empty plasmid pLVX-MYRF),and a TET1 overexpression group(transfected with the recombinant plasmid pLVX-MYRF-TET1).RT-qPCR and Western blot were used to detect the expression of TET1 mRNA and protein in HepG2 cells of each group.The proliferation ability was detected by CCK-8 assay.Transwell assay and cell scratch assay were used to de-tect the invasion and migration abilities.A subcutaneous tumor transplantation model was constructed to evaluate the tumor formation ability.Results:The TET1 overexpression group had significantly higher mRNA and protein levels of TET1 than blank group and the negative control group(all P＜0.05).The proliferation rates of cells in TET1 overexpression group at 24,48 and 72 hours were lower than those in blank group and negative control group(all P＜0.05).TET1 overexpression group had lower invasion and migration abilities than blank group and negative control group(all P＜0.05).The TET1 overexpression group had lower tumor volume and weight than blank group and negative control group(all P＜0.05).Conclusion:Overexpression of TET1 can inhibit the proliferation,in-vasion,migration of human liver cancer HepG2 cells,as well as the growth of liver cancer transplanted tumors in nude mice.