复合菌群的构建及其对马铃薯生长的影响OA

[Objective]This study aims to construct plant growth-promoting microbial consortia and systematically evaluate their efficacy in enhancing potato growth and their application potential under field conditions.[Method]Two efficient Ralstonia pickettii strains(Rp.CX15 and Rp.CX18)were selected as core components and combined with partner strains exhibiting growth-promoting(Serratia marcescens P2A12,Sm.P2A12;Pseudomonas sp.CX02,Ps.sp.CX02),salt-tolerant(Pseudomonas sp.P3A2,Ps.sp.P3A2),and pathogen-antagonistic(Pseudomonas chlororaphis P1E11,Pc.P1E11)traits.Strain compatibility was evaluated using metabolite-cell co-culture assays and plate confrontation tests.Fourteen groups of microbial consortia were initially screened in pot experiments to compare the efficacy of multi-strain consortia against individual strains.The growth-promoting ability of the multi-strain consortia was tested in a natural soil potted system,and growth-promoting effects under two inoculation methods were also compared.Field trials were subsequently conducted to evaluate the effect of the multi-strain consortia on tuber yield per plant.[Result]Four groups of effective consortia were identified:B2(Rp.CX18+Ps.sp.CX02),B4(Rp.CX18+Pc.P1E11),B6(Rp.CX18+Ps.sp.P3A2),and C4(Rp.CX18+Ps.sp.CX02+Ps.sp.P3A2).Under B2 treatment,stem diameter,aboveground dry weight,and belowground dry weight of potato seedlings increased by 50.37%,97.92%,and 117.85%,respectively.Substrate inoculation combined with seedling-stage inoculation(SSI)was more effective than substrate inoculation alone(SI).In field trials,B2 and B6 increased tuber weight per plant of diploid potato by 12.71% and 12.77%,respectively.[Conclusion]The microbial consortia B2 and B6,constructed with Rp.CX18 as the core strain,significantly enhanced potato seedling biomass and increased tuber weight per plant under field conditions.These findings provide a reference for the application of microbial consortia in potato and other crops.