基于高分辨率质谱的微生物宏代谢组解析方法研究进展OA

Elucidating the regulatory mechanisms and ecological functions of microbial metabolism is crucial for understanding the carbon biogeochemical cycling,predicting carbon-climate feedbacks,and modulating carbon flows in engineered applications.Microbial meta-metabolomics conceptualizes organic molecules as a"molecular community",enabling a holistic investigation into the composition and dynamics of metabolites at the community level.High-resolution mass spectrometry has emerged as a powerful approach for large-scale profiling of organic metabolites,and the resulting molecular fingerprints can be deeply explored by leveraging ecological analytical frameworks.However,challenges remain in linking metabolites to microbial functions and attributing metabolic processes.This review systematically summarizes recent methodological advances in meta-metabolomics,including the extended application of ecological mechanisms,the construction of molecular ecological networks,and multi-dimensional molecular profiling,while also highlighting current technical limitations.Looking forward,integrating multi-omics spatiotemporal analyses,identifying key metabolic roles,and extending ecological frameworks will be critical for establishing this field as a frontier in understanding ecosystem functions and carbon regulation.