青海东部农业区喜马拉雅旱獭肠道病毒种群宏基因组学分析OA

In recent years,zoonotic diseases have triggered several large-scale outbreaks of infectious diseases,highlight-ing the critical importance of prevention,detection,and diagnosis of zoonotic infections.The agricultural areas of Qing-hai are primarily concentrated in the eastern part of the province,where the habitat of Marmota himalayana partially overlaps with human activity areas.This study employed metagenomic sequencing technology to analyze and compare the composition and diversity of intestinal viruses,and their hosts in Marmota himalayana from six regions in eastern Qinghai's agricultural areas.The results of the study revealed that the predominant viral families were Caudovirales,Fictiviricetes,and Monodnaviria,with viruses at the order level mainly classified into Microvirales and Circovirales.At the family level,the major viral families were Microviridae and Viphaviridae.Most of the annotated viral genes were as-sociated with protein families involved in genetic information processing,signaling,cellular processes,as well as replica-tion and repair functions.Host prediction predominantly identified Escherichia coli from the Enterobacteriaceae family and Brevibacillus laterosporus from the Bacillaceae family as the main hosts.This study reveals the diversity and region-al differences of the virome in Marmota himalayana from the eastern agricultural region of Qinghai,suggesting that cer-tain viral families may exhibit geographic limitations and host specificity,providing a crucial foundation for future viral surveillance and pathogenicity research.