香菇多糖对攻毒猪流行性腹泻病毒断奶仔猪生长性能、排毒情况及血液指标的影响OA

The study aimed to investigate the effects of dietary supplementation with lentinan(LNT)on the growth performance,post-challenge diarrhea rate and virus shedding,and serum-related indicators of weaned piglets challenged with porcine epidemic diarrhea virus(PEDV).One hundred healthy 21-day-old weaned piglets of three-way crossbred with an average body weight of(6.10±0.64)kg were randomly divided into 4 groups with 25 piglets per group.The control group was fed a basal diet;the group 1 was fed a basal diet supplemented with 100 mg/kg LNT;the group 2 was fed a basal diet and challenged with PEDV on the 14th day of the experiment;the group 3 was fed a basal diet supplemented with 100 mg/kg LNT and challenged with PEDV on the 14th day of the experiment.The experimental period was 28 d.The growth performance,post-challenge diarrhea rate and virus shedding,and serum indicators of the weaned piglets were recorded and detected during the experiment.The results showed that dietary supplementation with lentinan and PEDV challenge had signifi-cant effects on final weight,average daily gain,average daily feed intake,and feed conversion ratio(P<0.05),and there was an interaction between the two(P<0.05).Compared with the control group,the final weight and average daily gain of the group 1 were significantly increased(P<0.05);the final weight,average daily gain,and average daily feed intake of the group 2 were significantly decreased(P<0.05),and the feed conversion ratio was significantly increased(P<0.05).On the 17th and 21st days after challenge,there was no significant difference in the diarrhea rate and the positive rate of antigen in feces between the group 2 and the group 3(P>0.05);on the 21st day,the Ct value of the group 3 was significantly higher than that of the group 2(P<0.05);on the 28th day,the diarrhea rate and the positive rate of antigen in feces of the group 3 were significantly lower than those of the group 2(P<0.05),and the Ct value was significantly higher(P<0.05).Dietary supplementation with lentinan had a significant effect on malondialdehyde content(P<0.05),and there was an interaction between dietary supplementation with lentinan and PEDV challenge(P<0.05).Compared with the control group,the urea nitrogen content of the group 1 was significantly decreased(P<0.05),and that of the group 2 was significantly increased(P<0.05).Compared with the control group,the malondialdehyde content of the group 2 was significantly increased(P<0.05).Dietary supplementation with lentinan and PEDV challenge had significant effects on superoxide dismutase activity(P<0.05),and there was an interaction between the two(P<0.05).Compared with the control group,the superoxide dismutase activity of the group 1 was significantly increased(P<0.05).Dietary supplementation with lentinan and PEDV challenge had significant effects on serum immunoglobulin G and immunoglobulin A content(P<0.05),and there was an interaction between the two(P<0.05).Compared with the control group,the immuno-globulin G of the group 1 was significantly increased(P<0.05),and the immunoglobulin A of the group 2 was significantly increased(P<0.05).In conclusion,dietary supplementation with LNT can play a positive role in the growth performance,antioxidant capacity,and immune indicators of weaned piglets,reduce the diarrhea rate of weaned piglets infected with PEDV,shorten the virus shedding period,and alleviate the decline in growth performance caused by PEDV infection.