益肾固胎颗粒通过调控Th17/Treg细胞平衡改善复发性流产的作用机制研究OA

Objective:To investigate the mechanism by which Yishen Gutai granules improve pregnancy out-comes in mice with recurrent spontaneous abortion(RSA)by regulating the Th17/Treg cell balance.Methods:Thirty CBA/J female mice were randomly divided into five groups:the control group,the normal pregnancy group,the RSA model group,the Yishen Gutai granules group,and the cyclosporine A group.From day 0 to day 14 of gestation,mice in the Yishen Gutai granules and cyclosporine A groups were administered 3.6 g/kg and 25 mg/kg,respectively,by gavage.After the final administration,placental pathology was observed via hematoxylin-eosin(HE)staining.Serum levels of interleukin-6(IL-6),interleukin-17α(IL-17α),transforming growth factor-β(TGF-β),and interleukin-10(IL-10)were detected by enzyme-linked immunosorbent assay(ELISA).The proportion of Th17 and Treg cells in peripheral blood was analyzed by flow cytometry.Results:Compared with the RSA model group,placental pathologi-cal injury in the Yishen Gutai granules group was significantly improved,with markedly alleviated inflammatory cell infiltration and tissue swelling.Serum levels of IL-6 and IL-17α decreased by 42.7％and 56.9％,respectively(P＜0.01),while expression levels of TGF-β and IL-10 increased by 2.1-fold and 1.7-fold,respectively.The proportion of Th17 cells decreased from(7.5±0.8)％to(3.2±0.5)％,the proportion of Treg cells increased from(8.2±0.9)％to(12.7±1.1)％,and the Th17/Treg ratio was restored from 0.32 to 0.25(P＜0.01).Conclusion:Yishen Gutai granules may improve pregnancy outcomes in RSA mice by regulating the Th17/Treg cell balance and enhan-cing immune tolerance at the maternal-fetal interface.These findings provide a molecular immunological basis for the traditional Chinese medicine method of"tonifying the kidney to secure the fetus".