针刺调控氧化应激对肥胖雄鼠生殖损伤的保护作用及机制研究OA

Objective:To investigate whether acupuncture ameliorates reproductive injury in obese male mice by regulating oxidative stress.Methods:Mice were randomly divided into a control group(n=18)and a high-fat diet group(n=35).The control group was fed a normal diet,while the high-fat diet group was fed a high-fat diet.After 10 weeks,body weight was measured and obese mice were screened.Successfully established obese mice were ran-domly divided into a model group and an acupuncture group(n=15).The acupuncture group received acupuncture intervention for 4 consecutive weeks.Sperm quality was evaluated.Serum levels of total cholesterol(TC),triglycer-ides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),testosterone(T),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were detected by ELISA.Testicular patho-logical morphology was observed by hematoxylin-eosin(HE)staining and Oil Red O staining.The contents of ma-londialdehyde(MDA),reduced glutathione(GSH),and total superoxide dismutase(T-SOD)in testicular tissue were measured by biochemical methods.Reactive oxygen species(ROS)levels in the testes were detected by flow cytome-try.Testicular mitochondrial structure was observed by transmission electron microscopy.The apoptosis rate of posi-tive cells in the testes was detected by TUNEL staining.Protein expression levels of tumor protein p53(p53),B-cell lymphoma-2(BCL-2),BCL-2-associated X protein(BAX),and cysteine protease-3(Caspase-3)were detected by Western blot.Results:Compared with the control group,the model group showed decreased sperm concentration and motility(P＜0.01,P＜0.05);increased serum TC,TG,and LDL-C levels(P＜0.01)and decreased HDL-C levels(P＜0.01);decreased serum T,FSH,and LH levels(P＜0.01);structural damage of seminiferous tubules in the testes with increased lipid deposition;increased MDA content(P＜0.01)and decreased GSH and T-SOD contents(P＜0.01);elevated ROS levels(P＜0.01);impaired mitochondrial morphology;increased apoptosis rate of positive cells(P＜0.01);decreased BCL-2 protein expression(P＜0.01);and increased expression of BAX,Caspase-3,and p53 proteins(P＜0.01).Compared with the model group,the acupuncture group showed increased sperm concentra-tion and motility(P＜0.01,P＜0.05);increased serum HDL-C levels(P＜0.01)and decreased TC,TG,and LDL-C levels(P＜0.01,P＜0.05);increased T,FSH,and LH levels(P＜0.01,P＜0.05);improved testicular pathological morphology and reduced lipid deposition;decreased MDA content(P＜0.01)and increased GSH and T-SOD con-tents(P＜0.01,P＜0.05);reduced ROS levels(P＜0.01);improved mitochondrial number and morphology;de-creased apoptosis rate of positive cells(P＜0.01);increased BCL-2 protein expression(P＜0.05);and decreased BAX,Caspase-3,and p53 protein expression(P＜0.01,P＜0.05).Conclusion:Acupuncture can regulate oxidative stress levels,improve mitochondrial function,and inhibit excessive apoptosis of spermatogenic cells,thereby impro-ving obesity-related sperm quality decline.