一种保湿舒缓修护功效组合物及其应用研究OA

A functional composition with moisturizing,soothing,and repairing properties,based on glucosyl-glycerol and a Lactobacillus/algae extract fermentation product,was developed and incorporated into a stable emulsion system.Its efficacy was systematically evaluated using multidimensional models,including in vitro moisturizing assays,anti-inflammatory assays in HaCaT cells,hyaluronidase inhibition,the hen's egg chorioallantoic membrane(HET-CAM)test,HaCaT cell scratch assay,and human efficacy studies.The results demonstrated that the combination of glucosyl-glycerol and the Lactobacillus/algae extract fermentation product exhibited superior anti-erythema effects compared with either component alone in a histamine-induced skin erythema model.The emulsion containing this composition showed significantly higher skin hydration than the control group at 2 and 6 h(p<0.01),along with significantly reduced trans-epidermal water loss(TEWL)(p<0.01),indicating rapid hydration and sustained moisture retention.The formulation significantly inhibited IL-6 expression(p<0.05)and exhibited a hyaluronidase inhibition rate of 61.15%±5.02%,suggesting effective suppression of inflammatory cascades and hyaluronic acid degradation.The HET-CAM assay confirmed that the formulation was mild and non-irritating.In the HaCaT cell scratch assay,the relative cell migration rate in the emulsion group reached 157.13%compared with the control group(p<0.01),indicating enhanced wound-healing capacity.In a 14-day human study,stratum corneum hydration increased by 19.95%,hemoglobin content decreased by 19.01%,and TEWL decreased by 16.80%.Overall,the emulsion containing this functional composition demonstrated excellent moisturizing,soothing,and repairing properties,and effectively strengthened the skin barrier.