侧柏枝挥发油促进雄激素性脱发小鼠毛发生长研究OA

Supercritical CO2 extraction was employed to isolate the essential oil from Platycladus orientalis L.branches(EOPB).The extraction process was optimized using a Box-Behnken design(BBD)with the extraction yield as the response variable.The chemical constituents of the extracted essential oil were characterized via gas chromatography-mass spectrometry.An androgenetic alopecia(AGA)model was established in C57BL/6 mice by depilating the dorsal hair and administering daily intraperitoneal injections of testosterone to evaluate the hair growth-promoting effects of EOPB.The underlying mechanisms were investigated using network pharmacology.Furthermore,the cutaneous levels of IL-1β,IL-6,TNF-α,SOD,CAT,and MDA were measured using ELISA kits.The results indicate that the optimal extraction parameters for EOPB are a pressure of 10.62 MPa,a temperature of 43.69℃,and an extraction time of 83.86 minutes,achieving a maximum extraction yield of 20.107%.The primary constituents of EOPB are identified as thujopsene(32.27%)and cedrol(20.88%).The 10%(φ/%)EOPB group achieves complete hair regrowth by day 21,exhibiting an efficacy comparable to that of the minoxidil positive control.Histological analysis confirms that EOPB enhances hair follicle density and accelerates the anagen phase transition.Network pharmacology analysis reveals the involvement of EOPB in inflammatory and oxidative stress pathways.EOPB effectively suppresses inflammatory factors(IL-1β,IL-6,TNF-α),restores antioxidant enzyme(SOD and CAT)activities,and reduces lipid peroxidation(MDA levels).This study provides a reference for the application of EOPB in hair care products,offering alternative therapeutic choices for patients with androgenetic alopecia.