沉默GhMLO6基因可增强棉花对黄萎病的抗性OA

[Objective]This study aimed to investigate the function of the GhMLO6 gene in cotton Verticillium wilt resistance and identify a candidate gene for disease resistance breeding in cotton.[Methods]Quantitative real-time polymerase chain reaction(qRT-PCR)was used to analyze the expression pattern of GhMLO6.The virus-induced gene silencing(VIGS)technique was employed to transiently silence GhMLO6 in cotton to study its function in Verticillium wilt resistance.Agrobacterium-mediated transient expression technology was applied to Nicotiana benthamiana to explore the subcellular localization of GhMLO6.[Results]The qRT-PCR results revealed that GhMLO6 presented the highest expression level in cotton roots,followed by stems and leaves.Compared with the control,infection with Verticillium dahliae significantly increased the expression level of GhMLO6 in roots.Compared with the control plants in which empty vectors were used,GhMLO6-silenced plants displayed a significant decrease in disease index and relative fungal DNA content.After stem recovery and cultivation,the accumulation of hyphae was reduced,and the resistance of cotton to Verticillium wilt was significantly increased.Transient expression analysis indicated that GhMLO6 localizes on the cell membrane.[Conclusion]GhMLO6 is localized to the cell membrane and performs a negative regulatory function in cotton Verticillium wilt resistance.Therefore materials with Verticillium wilt resistance could be obtained via gene editing technology.