不同品系德州驴盲肠微生物组成的比较研究OA

This study aims to investigate the differences in the microbial composition of the cecum a-mong different breeds of Dezhou donkeys.A total of 20 healthy male donkeys,aged 11 months and weig-hing(185±3)kg,were randomly selected and divided into two groups:the Sanfen donkey group(n=10)and the Wutou donkey group(n=10),10 donkeys in each group.Both groups were fed a pelleted total mixed ration(TMR)with the same formulation and nutritional level for a pre-feeding period of 14 days,followed by a formal feeding period of 86 days,totaling 100 days.After the feeding trial,the donkeys were slaughtered,and the contents of the cecum were collected to analyze the microbial composition.Results showed that(1)a total of 29 phyla,51 classes,114 orders,180 families,and 265 genera were identified.(2)The alpha diversity indices-including Chao,ACE,Shannon,and Simpson-showed no significant differ-ence between the Sanfen and Wutou donkeys(P＞0.05),indicating that the microbial richness and diversi-ty were similar.The Principal Coordinates Analysis(PCoA)plot demonstrated partial overlap between the two groups,but no clear separation was observed.(3)At the phylum level,both breeds predominantly harbored Firmicutes and Bacteroidota,accounting for more than 75％relative abundance.At the genus level,the top five genera in the Sanfen donkey,ranked from high to low,were Phascolarctobacterium,Ruminococcaceae_UCG-005,Alloprevotella,Streptococcus,and Akkermansia;in the Wutou donkey,the top five genera were Christensenellaceae_R-7_group,Methanobrevibacter,Streptococcus,Rikenellaceae_RC9_gut_group,and Akkermansia.The F/B ratio(the ratio of Firmicutes to Bacteroidota)was numeri-cally higher in Wutou donkeys but did not reach significant differences(P＞0.05).(4)At the phylum lev-el,there were three differentially expressed microorganisms,and at the genus level,there were nine differentially expressed microorganisms.In summary,the microbial community in the cecum of Dezhou donkeys is complex and diverse;there were no significant differences in microbial richness and diversity between the two breeds,and the PCoA analysis revealed that their microbial community structures are largely similar,with overall minimal differences.