坝上长尾鸡腿肌脂肪酸含量相关基因多态性及其关联分析OA

This study investigated the association between fatty acid content in the thigh muscle of Bas-hang Long-tailed chickens and their genotypes.The experimental samples were divided into high-fat and low-fat groups(each consisting of 20 individuals).Whole-genome sequencing was performed on 61 chick-ens,with sequencing depths exceeding 10 ×.Genetic differentiation between the high-and low-fat groups was analyzed using FST statistics to identify genomic regions under selection.Candidate genes related to fat synthesis and metabolism were identified through functional annotation,Gene Ontology(GO),and KEGG pathway enrichment analyses.The results showed that the average fatty acid content in the high-fat group was 8.866±2.52 g/100 g,while in the low-fat group it was 2.3885±0.45 g/100 g.Based on a sliding win-dow analysis of FST,a total of 16,333 selective sweep regions were identified,involving 889 genes.GO en-richment analysis indicated that the differentially expressed genes were significantly overrepresented in pathways related to adipocyte differentiation;KEGG pathway analysis revealed that genes such as BCAT1,PDGFRA,MAP3K5,ANGPT1,SCD,and ACOX3 were significantly associated with fatty acid metabolism.PCR sequencing validation demonstrated that exonic regions of MAP3K5,BCAT1,PDGFRA,and ANGPT1 were significantly correlated with fatty acid content(P＜0.05).The candidate genes and their non-synonymous mutation sites identified in this study elucidate the genetic mechanisms underlying fat deposition in Bashang Long-tailed chickens and provide valuable molecular markers for the genetic improvement of fat-related traits in poultry.