circFOXP1靶向miR-4429调控鼻咽癌6-10B细胞的增殖、迁移及侵袭OA

Objective To determine whether circular RNA forkhead box protein P1(circFOXP1)regulates the proliferation,migration and invasion of nasopharyngeal carcinoma 6-10B cells by targeting miR-4429.Methods The correlations of circFOXP1 and miR-4429 with pathological characteristics of nasopharyngeal carcinoma patients were analyzed.Nasopharyngeal carcinoma 6-10B cells were transfected with si-NC,si-circFOXP1,pcDNA,pcDNA-circFOXP1,miR-NC,miR-4429 mimics,or co-transfected with si-circFOXP1 and anti-miR-NC,or si-circFOXP1 and anti-miR-4429.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect circFOXP1 and miR-4429 expression in nasopharyngeal carcinoma tissues.Cell viability,colony formation,migration and invasion abilities were assessed by Cell Counting Kit-8(CCK-8),colony formation,wound healing and Transwell assays.Western blotting was used to detect E-cadherin and N-cadherin protein expression.A dual-luciferase reporter assay verified the targeting relationship between circFOXP1 and miR-4429.Results The expression level of circFOXP1 in nasopharyngeal carcinoma tissues(4.61±0.31)was significantly higher than that in adjacent tissues(1.00±0.05)(P<0.001),while the expression level of miR-4429(0.37±0.03)was significantly lower than that in adjacent tissues(1.00±0.08)(P<0.001).The expression of miR-4429 was correlated with tumor volume and TNM stage(P<0.05),while the expression of circFOXP1 was correlated with TNM stage(P<0.05)but not with tumor volume(P>0.05).Inhibition of circFOXP1 expression or overexpression of miR-4429 reduced cell viability,colony formation number,wound healing rate,invasive cell number and N-cadherin protein expression,while increased E-cadherin protein expression in nasopharyngeal carcinoma 6-10B cells(P<0.05).circFOXP1 targeted and negatively regulated miR-4429 expression.Interference with miR-4429 reversed the inhibitory effects of circFOXP1 silencing on proliferation,migration and invasion of nasopharyngeal carcinoma cells(P<0.05).Conclusion Inhibition of circFOXP1 expression can attenuate the proliferation,migration and invasion of nasopharyngeal carcinoma 6-10B cells by targeting miR-4429.