席夫碱探针可视化淬灭识别铜离子和铁离子OA

To achieve rapid and specific detection of Cu2+and Fe3+in complex environments,we designed and synthesized a novel Schiff base fluorescent probe based on 2-furancarboxyhydrazide.Target probe 1 was successfully obtained by introducing 4-dimethylaminocinnamaldehyde into the amino site of 2-furancarboxaldehyde hydrazide,and its structure was comprehensively characterized using 1 H and 13 C nuclear magnetic resonance(NMR)spectroscopy and infrared spectroscopy.The recognition performance of probe 1 for various metal ions was investigated using UV-visible absorption spectroscopy and fluorescence spectroscopy.The results showed that in a system with multiple cations,probe 1 achieved rapid and highly sensitive quenching recognition of Cu2+and Fe3+with good selectivity.Job's plot indicated that the complexation ratio of probe 1 with both ions was 1∶1.By combining NMR titration with density functional theory calculations,the binding sites of the probe and ions,as well as the fluorescence quenching mechanism,were elucidated.Based on this,probe 1 was combined with a portable 365 nm ultraviolet lamp to develop a simple strip-based platform for ion sensing.This platform enables the rapid on-site identification of Cu2+and Fe3+by observing the degree of fluorescence quenching with the naked eye.In summary,the novel Schiff base fluorescent probe obtained in this study exhibits high selectivity and sensitivity towards Cu2+and Fe3+.Combined with a portable test strip platform,the probe provides a feasible solution for rapid on-site detection of metal ions.