UHPLC-HRMS结合AntDAS剖析不同产地金银花特征物质及产地溯源研究OA

The chemical compositions of Lonicerae japonicae Flos samples from four producing areas—Fengqiu,Henan;Xinmi,Henan;Pingyi,Shandong and Zhongwei,Ningxia—were analyzed,and a geographic origin traceability model was established to provide technical support for the authentic identification of Lonicerae japonicae Flos and the standardized development of the Lonicerae japonicae Flos industry.Ultrahigh-performance liquid chromatography-high-resolution mass spectrometry(UHPLC-HRMS)and the data analysis software AntDAS-DDA were used for compound data extraction,alignment,screening of differential components,and compound identification.Geographic origin discrimination models constructed using four classical chemometric methods were evaluated based on the screened differential metabolites.A total of 62 differential metabolites belonging to 11 categories,including flavonoids,coumarins,and phenolic acids,were screened and identified.The partial least-squares discriminant analysis model,constructed based on the differential metabolites,exhibited excellent goodness of fit and predictive ability(R2>0.9,Q2>0.8)and accurately distinguished Lonicerae japonicae Flos samples from the four producing areas,with a model accuracy of 98.60%.Significant differences were found in differential metabolites of Lonicerae japonicae Flos from different producing regions.Lonicerae japonicae Flos from Xinmi area had higher flavonoid contents,while Fengqiu-derived samples were richer in terpenoids.The established origin discrimination model could effectively differentiate sample origins.These results are expected to facilitate the standardized development of the Loniceras japonicas Flos industry in China.