甘草查尔酮A改善黄曲霉毒素B1诱导的雏鸡肝脏脂质代谢异常的作用研究OA

In order to investigate the effect of licochalcone A(Lico A)on abnormal liver lipid metabolism induced by aflatoxin B1(AFB1)in chicks,80 one-day-old Arbor Acres roosters were adaptively fed for 3 days and then randomly divided into four groups(20 each group):the control group,Lico A group(50 mg/kg by feed weight),AFB1 group(2 mg/kg by feed weight),and Lico A(50 mg/kg by feed weight)+AFB1(2 mg/kg by feed weight)group.The chicks were fed continuously for 21 days.At the end of the experiment,growth performance indicators and blood lipid indicators were measured.After the chicks were sacrificed,liver tissues were collected for oil red O staining,transcriptomic analysis,and detection of lipid metabolism-related protein expression.The results showed that compared with the control group,the average daily weight gain in AFB1 group was significantly decreased(P<0.05).Compared with the AFB1 group,the average daily weight in Lico A+AFB1 group was extremely significantly increased(P<0.01).No red lipid droplet deposits were observed in the control group and Lico A group,while in AFB1 group,lipid droplets were deeply stained and larger in size.In Lico A+AFB1 group,lipid droplet staining became lighter,and both the number and volume of lipid droplets decreased.Compared with the control group,the mass concentrations of triglycerides(TG),total cholesterol(T-CHO),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C)in the serum of AFB1 group were extremely significantly increased(P<0.01).Compared with AFB1 group,the mass concentrations of TG,T-CHO,LDL-C in Lico A+AFB1 group were significantly decreased(P<0.05),the mass concentration of HDL-C was extremely significantly decreased(P<0.01).Compared with the control group,the expression levels of lipid metabolism-related genes PPARA and PPARGC1A were extremely significantly upregulated in AFB1 group(P<0.01),and lipid metabolism pathways were altered,the relative expressions of fatty acid oxidation-related proteins peroxisome proliferator-activated receptor gamma coactivator 1-α(PGC-1α)and peroxisome proliferator-activated receptor α(PPARα)in the liver was significantly increased in the AFB1 group(P<0.05),while the relative expression of fatty acid synthesis-related proteins 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCR),sterol regulatory element-binding transcription factor 1(SREBP1),and fatty acid synthase(FAS)was extremely significantly decreased(P<0.01).Compared with AFB1 group,the relative expression of FAS in Lico A+AFB1 group was significantly decreased(P<0.05).It indicated that Lico A alleviates AFB1-induced liver lipid damage in chicks through multiple pathways,such as improving the lipid metabolism disorder in the liver caused by AFB1 and regulating key lipid metabolism signaling pathways.