水温与溶解氧协同作用对斑鳠胚胎发育的影响OA

To investigate the synergistic effects of water temperature and dissolved oxygen(DO)on the embryonic development of Mystus guttatus,a controlled-environment incubation experiment was conducted in Luokun Town,Luodian County,Guizhou Province,from August 2 to 4,2025.The control group was set at a DO level simulating natural river water(4.0±0.5)mg/L,while two oxygen-enhanced groups were established:Group 1(5.5±0.5)mg/L and Group 2(7.0±0.5)mg/L.Within each DO group,five parallel temperature gradients(25℃,26℃,27℃,28℃,29℃)were arranged,with 3 000 embryos per replicate.The results showed that the average hatch-ing rates of the control group,Group 1 and Group 2 were 12.11%,18.87%and 31.21%,respectively.The average hatching rates of both experimental groups were extremely significantly higher than that of the control group(P<0.01),with consistent significant increases at all temperature gradients(P<0.01).The peak hatching rate of 40.03%was achieved at DO(7.0±0.5)mg/L and water temperature(28±1)℃.This study demonstrated that wa-ter temperature and DO exerted significant synergistic effects on the embryonic development of Mystus guttatus.In-creasing DO level and precisely controlling suitable water temperature can greatly improve the embryonic hatching efficiency of Mystus guttatus,with the optimal hatching conditions determined as 7 mg/L DO and 28℃water tem-perature.