hsa_circ_0005245调控miR-873-5p/STMN1轴对甲状腺癌生长和转移的作用机制OA
The Mechanism of hsa_circ_0005245 Regulating miR-873-5p/STMN1 Axis on the Growth and Metastasis of Thyroid Cancer
目的:探究 hsa_circ_0005245 调控微小 RNA-873-5p(miR-873-5p)/抑微管装配蛋白 1(STMN1)轴对甲状腺癌生长和转移的影响.方法:用 qRT-PCR 法检测甲状腺癌细胞系(KTC-1、TPC-1、C643)和组织中 hsa_circ_0005245、miR-873-5p、STMN1 mRNA 水平;双荧光素酶实验检测 STMN1 与miR-873-5p、hsa_circ_0005245 与 miR-873-5p 的结合关系;将 KTC-1 细胞分为 Control 组、sh-NC、sh-0005245、sh-0005245+anti-NC、sh-0005245+anti-miR-873-5p;检测各组 KTC-1 细胞增殖、迁移与侵袭情况.Western Blot 和免疫组化分别检测 MMP-2、MMP-9、PCNA、上皮间质转化(EMT)(N-Cadherin、Vimentin、E-Cadherin)与 STMN1 蛋白表达.结果:甲状腺癌组织以及癌细胞系(TPC-1、KTC-1、C643细胞)中 hsa_circ_0005245、STMN1 mRNA 表达较高,miR-873-5p 表达趋势相反(P<0.05).StarBase 数据库显示,hsa_circ_0005245 与 miR-873-5p、STMN1 与 miR-873-5p 分别存在结合关系.与 sh-NC 组比较,sh-0005245 组 hsa_circ_0005245、STMN1、PCNA、N-Cadherin、Vimentin、MMP-2、MMP-9 下调表达,miR-873-5p、E-Cadherin 上调表达,KTC-1 细胞 Edu 阳性率、迁移数、侵袭数目降低(P<0.05);与sh-0005245+anti-NC 组比较,sh-0005245+anti-miR-873-5p 组 miR-873-5p、E-Cadherin 表达下降,PCNA、MMP-2、MMP-9、N-Cadherin、Vimentin、STMN1 表达上升,Edu 阳性率、细胞迁移数、侵袭细胞数目上升(P<0.05).sh-0005245 组肿瘤体积、重量比 sh-NC 组小,hsa_circ_0005245、STMN1 表达比 sh-NC 组低,miR-873-5p 表达比 sh-NC 组高(P<0.05).结论:抑制 hsa_circ_0005245 表达可以靶向 miR-873-5p/STMN1 轴抑制甲状腺癌细胞增殖、迁移、侵袭、EMT 等生物学行为.
Objective:To explore the effect of hsa_circ_0005245 on the growth and metastasis of thyroid cancer by regulating the microRNA-873-5p(miR-873-5p)/stathmin 1(STMN1)axis.Methods:QRT-PCR was used to detect the mRNA levels of 0005245,miR-873-5p,and STMN1 in thyroid cancer cell lines(KTC-1,TPC-1,C643)and tissues.A dual-luciferase assay was used to examine the binding interactions between STMN1 and miR-873-5p,and between hsa_circ_0005245 and miR-873-5p;KTC-1 cells were as-signed into Control group,sh-NC group,sh-0005245 group,sh-0005245+anti-NC group,sh-0005245+an-ti-miR-873-5p group,the proliferation,migration,and invasion of KTC-1 cells in each group were detec-ted.Western blot and immunohistochemistry were used to detect the expression of MMP-2,MMP-9,PCNA,epithelial-mesenchymal transition(EMT)markers(N-cadherin,vimentin,E-cadherin)and STMN1 Protein Expression.Results:The mRNA expression of hsa_circ_0005245 and STMN1 increased in thyroid cancer tis-sues and thyroid cancer cell lines(KTC-1,TPC-1,C643 cells),while the expression of miR-873-5p de-creased(P<0.05).The StarBase database showed a binding relationship between hsa_circ_0005245 and miR-873-5p,and between STMN1 and miR-873-5p.Compared with the sh-NC group,the expression of hsa_circ_0005245,STMN1,PCNA,MMP-2,MMP-9,N-Cadherin,and Vimentin decreased in the sh-0005245 group,the expression of miR-873-5p and E-Cadherin increased,and the Edu positivity rate,cell migration number,and invasive cell number decreased(P<0.05).Compared with the sh-0005245+anti-NC group,the expression of miR-873-5p and E-Cadherin decreased in the sh-0005245+anti miR-873-5p group,the expression of PCNA,MMP-2,MMP-9,N-Cadherin,Vimentin,and STMN1 increased,and the Edu positivity rate,cell migration number,and invasive cell number increased(P<0.05).Tumor volume and weight were smaller in the sh-0005245 group than sh-NC group,hsa_circ_0005245 and STMN1 expression was lower,and miR-873-5p expression was higher than sh-NC group(P<0.05).Conclusion:Inhibition of hsa_circ_0005245 expression can target the miR-873-5p/STMN1 axis to suppress biological behaviours in thyroid cancer cells,including proliferation,migration,invasion,and EMT.
陆皓东;郭欣;杜静海;李辉;孙文滨;甄艳
河北省唐山中心医院甲状腺头颈颌面外科,河北 唐山 063000河北省唐山中心医院甲状腺头颈颌面外科,河北 唐山 063000河北省唐山中心医院甲状腺头颈颌面外科,河北 唐山 063000河北省唐山中心医院甲状腺头颈颌面外科,河北 唐山 063000河北省唐山中心医院甲状腺头颈颌面外科,河北 唐山 063000河北省唐山中心医院甲状腺头颈颌面外科,河北 唐山 063000
甲状腺癌hsa_circ_0005245微小 RNA-873-5p/抑微管装配蛋白1轴生长转移
Thyroid cancerhsa_circ_0005245MicroRNA-873-5p/Stathmin 1 axisGrowthTransfer
《河北医学》 2026 (5)
729-737,9
河北省2023年度医学科学研究课题计划项目(编号:20231772)
评论