LncRNA LAMTOR5-AS1调节miR-873-5p/E2F3信号轴对胃癌细胞恶性生物学行为的影响OA
Effect of LncRNA LAMTOR5-AS1 on Malignant Biological Behaviors of Gastric Cancer Cells by Regulating the miR-873-5p/E2F3 Signaling Axis
目的:探讨长链非编码 RNA(LncRNA)LAMTOR5-AS1 调节微小 RNA-873-5p(miRNA-873-5p)/E2F 转录因子3(E2F3)信号轴对胃癌(GC)细胞恶性生物学行为的影响.方法:qRT-PCR 法检测各组 GC 细胞系中 LncRNA LAMTOR5-AS1、miR-873-5p、E2F3 mRNA 的表达;双荧光素酶报告基因实验验证 LncRNA LAMTOR5-AS1 与 miR-873-5p、miR-873-5p 与 E2F3 的互作.将 AGS 细胞随机分为 Ctrl 组、sh-NC 组、sh-LAMTOR5-AS1 组、sh-LAMTOR5-AS1+anti-NC 组、sh-LAMTOR5-AS1+anti-miR-873-5p 组,qRT-PCR 检测各组细胞中 mRNA 的表达;MTS 法和平板克隆实验检测 AGS 细胞增殖;划痕实验检测 AGS 细胞迁移;Transwell 实验检测 AGS 细胞侵袭;Western blot 检测 AGS 细胞中MCM2、E2F3、Snail、Bad 蛋白的表达.结果:GC 细胞系 LncRNA LAMTOR5-AS1、E2F3 mRNA 表达升高,miR-873-5p 表达降低(P<0.05).sh-LAMTOR5-AS1 组克隆数、OD490 值、划痕愈合率、侵袭数、Ln-c RNA LAMTOR5-AS1、E2F3 mRNA 和蛋白、MCM2、Snail 低于 sh-NC 组、Ctrl 组,凋亡率、miR-873-5p、Bad 高于 sh-NC 组、Ctrl 组(P<0.05);sh-LAMTOR5-AS1+anti-miR-873-5p 组克隆数、OD490 值、划痕愈合率、侵袭数、E2F3 mRNA 和蛋白、MCM2、Snail 高于 sh-LAMTOR5-AS1 组、sh-LAMTOR5-AS1+anti-NC 组,凋亡率、miR-873-5p、Bad 表达低于 sh-LAMTOR5-AS1 组、sh-LAMTOR5-AS1+anti-NC 组(P<0.05).LncRNA LAMTOR5-AS1 可以靶向负调控 miR-873-5p,miR-873-5p 可以靶向负调控 E2F3.结论:敲低 LncRNA LAMTOR5-AS1 可以通过调节 miR-873-5p/E2F3 信号轴,抑制 GC 细胞恶性生物学行为.
Objective:To discuss the effect of long non-coding RNA(LncRNA)LAMTOR5-AS1 on the malignant biological behaviors of gastric cancer(GC)cells by regulating the microRNA-873-5p(miRNA-873-5p)/E2F transcription factor 3(E2F3)signaling axis.Methods:The expression levels of LncRNA LAMTOR5-AS1,miR-873-5p and E2F3 mRNA in GC cell lines of each group were detected by qRT-PCR.The dual-luciferase reporter gene assay was used to verify the interactions between LncRNA LAMTOR5-AS1 and miR-873-5p,as well as between miR-873-5p and E2F3.AGS cells were randomly assigned into the Ctrl group,sh-NC group,sh-LAMTOR5-AS1 group,sh-LAMTOR5-AS1+anti-NC group,and sh-LAM-TOR5-AS1+anti-miR-873-5p group.The expression of mRNA in cells of each group was detected by qRT-PCR.The proliferation of AGS cells was detected by MTS assay and colony formation assay.The scratch assay was performed to measure the migration of AGS cells.Transwell assay was performed to detect AGS cells inva-sion.The protein expression of MCM2,E2F3,Snail and Bad in AGS cells was measured by Western blot.Results:The expression of LncRNA LAMTOR5-AS1 and E2F3 mRNA in the GC cell line was raised,while the expression of miR-873-5p decreased(P<0.05).Compared with the sh-NC group and the Ctrl group,the sh-LAMTOR5-AS1 group showed lower clone number,OD490 value,scratch healing rate,invasion num-ber,the expression of LncRNA LAMTOR5-AS1,E2F3(both mRNA and protein),MCM2,and Snail,while the apoptosis rate,miR-873-5p and Bad were notably higher(P<0.05).Compared with the sh-LAMTOR5-AS1 group and the sh-LAMTOR5-AS1+anti-NC group,the sh-LAMTOR5-AS1+anti-miR-873-5p group showed higher clone number,OD490 value,scratch healing rate,invasion number,E2F3(both mRNA and protein),MCM2,and Snail,while the apoptosis rate,miR-873-5p and Bad were notably lower(P<0.05).LncRNA LAMTOR5-AS1 was able to target and negatively regulate miR-873-5p,and miR-873-5p was able to target and negatively regulate E2F3.Conclusion:Knockdown of LncRNA LAMTOR5-AS1 can inhibit the malignant biological behaviors of GC cells by regulating the miR-873-5p/E2F3 signaling axis.
陈碧薇;侯倩倩;郑岳林;李辰
河北省衡水市人民医院,河北 衡水 053000河北省衡水市人民医院,河北 衡水 053000河北省衡水市人民医院,河北 衡水 053000天津中医药大学第二附属医院肿瘤内科,天津 300250
胃癌长链非编码 RNALAMTOR5-AS1微小 RNA-873-5pE2F 转录因子3细胞恶性生物学行为
Gastric cancerLong non-coding RNALAMTOR5-AS1MicroRNA-873-5pE2F transcription factor 3Cell malignant biological behaviors
《河北医学》 2026 (5)
713-720,8
河北省医学科学研究课题计划项目(编号:20261160)
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