木犀草素功能性脂质体制备处方工艺优化及其对Hep3B细胞的影响OA

Objective:To optimize the preparation process of transferrin modified functional liposomes of magnolol and to investigate their effects on Hep3B cells.Methods:Transferrin-modified luteolin-loaded liposomes(TF-LUT@LIP)was synthesized using the thin-film dispersion method.The encapsulation efficiency served as the evaluation criterion.Optimal preparation conditions were determined through single-factor experiments and response surface optimization.The effects on Hep3B cell viability and uptake were assessed via CCK-8 assays and cellular uptake experiments.Results:The optimal formulation preparation conditions are as follows:bile-to-lipid ratio of 1∶6.906,drug-to-lipid ratio of 1∶21.267,ultrasonication power of 38%(total power 650 W),and hydration temperature of 50℃.The response surface methodology-optimized luteolin liposomes achieved an average encapsulation efficiency of 96.94%.When the target molecule loading was 3%,the prepared TF-LUT@LIP exhibited(95.31±0.03)%,an average particle size of(184.53±0.61)nm,a zeta potential of(-23.800±0.500)mV,and excellent stability.TF-LUT@LIP exhibited stronger concentration-dependent inhibitory effects on Hep3B cells than free luteolin(LUT).Compared to non-targeted liposomes,TF-LUT@LIP demonstrated significantly enhanced uptake behavior.Conclusion:TF-LUT@LIP prepared via the optimal synthesis process exhibits high encapsulation efficiency,uniform morphology,and consistent particle size distribution.It demonstrates significant inhibitory effects on Hep3B cells and exhibits favorable cellular uptake behavior.