静脉麻醉药物丙泊酚对人单核细胞诱导小胶质细胞样细胞自发钙振荡的影响OA

Objective To establish a human monocyte-derived microglia-like cell(MDMi)model,character-ize its spontaneous calcium oscillatory activity,and investigate the effects of the anesthetic agent propofol on calcium signa-ling in MDMi.Methods Peripheral blood monocytes were isolated and differentiated into MDMi over 14 days using cyto-kine induction.Cellular identity was validated through morphological assessment,immunofluorescence staining,and in-flammatory cytokine release following lipopolysaccharide(LPS)stimulation.Calcium imaging was employed to record spontaneous calcium transients,and the effects of propofol at different concentrations(25-200 μmol/L)on oscillation frequency and amplitude were analyzed.Results MDMi cells with typical ramified morphology were successfully genera-ted,exhibiting high expression of microglia-specific markers IBA1 and TMEM119.These cells responded robustly to LPS stimulation,with approximately a fivefold increase in TNF-α secretion.Calcium imaging revealed active spontaneous cal-cium oscillations under resting conditions.Propofol modulated calcium activity in a dose-dependent manner:compared with the control group,50 μmol/L and 100 μmol/L propofol significantly increased both the frequency and amplitude of calcium oscillations,whereas 200 μmol/L propofol suppressed these parameters.Conclusion MDMi exhibit key pheno-typic and functional characteristics of human microglia.Propofol exerts bidirectional,concentration-dependent effects on calcium signaling in MDMi,enhancing oscillatory activity at clinically relevant concentrations while inhibiting it at higher concentrations.