炭疽芽孢生物标志物2,6-吡啶二羧酸检测技术的研究进展OA

Anthrax is a severe zoonotic infectious disease caused by Bacillus anthracis,whose spores possess extremely strong infectivity and environmental resistance,posing a major threat to public health and biosecurity.As a unique biomarker of anthrax spores,trace and highly specific detection of 2,6-pyridinedicarboxylic acid(DPA)is crucial for early diagnosis of anthrax,epidemic early warning,and biosecurity prevention and control.In recent years,DPA chemical detection techniques have become a research hotspot in this field due to their advantages of simple operation,low cost,high sensitivity and rapid response.Firstly,this review briefly introduced the research status of biological detection techniques for Bacillus anthracis and its spores,including polymerase chain reaction,loop-mediated isothermal amplification and CRISPR-Cas techniques.Subsequently,it focused on summarizing the latest research progress of DPA detection based on chemical techniques,including principles,advantages,disadvantages,and differences in limit of detection of optical,electrochemical and hyphenated detection techniques.Finally,combined with the current technical bottlenecks,future optimization directions and application prospects of DPA detection technologies were prospected.