基于丝素蛋白-铂纳米酶的可穿戴电化学传感贴片用于汗液中尿酸的检测OA

To meet the daily monitoring demand of uric acid(UA)in sweat,a novel wearable electrochemical sensing patch was constructed for highly sensitive detection of UA.The patch integrated platinum nanozyme with uricase-like activity,synthesized in situ using silk fibroin(SF)as a reducing and stabilizing agent,with a flexible laser-induced graphene(LIG)electrode.Leveraging the reducing capability of amino acid residues in SF,platinum nanoparticles were uniformly grown in situ.Modification of the LIG electrode surface with this composite yielded an integrated ″catalytic center-conductive substrate″ architecture,which not only increased the number of active sites but also facilitated the interfacial electron transfer.Electrochemical characterization revealed that the fabricated sensing patch exhibited a distinct UA oxidation peak at approximately 0.4 V.The peak current demonstrated a good linear relationship with UA concentration in the range of 0.1‒200 μmol/L,with a detection limit(S/N=3)as low as 0.03 μmol/L.Furthermore,the patch displayed excellent selectivity for UA,effectively eliminating interference from common coexisting substances such as glucose and urea.In simulated sweat samples,the recovery rates for spiking UA ranged from 101.6%to 104.7%.Critically,when applied to direct detection of UA in authentic human sweat,the patch validated its outstandingin vivodetection performance.This study developed a novel electrochemical sensing strategy based on uricase-like activity of Pt-SF,offering a viable pathway for the non-invasive,highly sensitive analysis of UA in sweat.