猪组织蛋白酶H与猪非典型瘟病毒Mut蛋白相互作用研究OA

In order to explore the interaction between Cathepsin H(CTSH)and Mut protein of Atypical Porcine pestivirus-Yunnan(APPV-YN)and its effect on APPV replication,the physicochemical properties and subcellular localization of CTSH gene expression protein were analyzed and predicted by online soft-ware such as ProtParam.The interaction between CTSH and APPV-YN Mut in vitro and in vivo was veri-fied by GST pull-down and Bimolecular fluorescence complementation(BiFC),respectively.The effects of overexpression or knockdown of host protein CTSH on APPV replication were determined by qPCR and Western blot.The results showed that CTSH protein was a hydrophilic stable protein,the secondary struc-ture was mainly random coil,and the tertiary structure was stable,which may be mainly located in the cy-toplasm.Mut interacts with the host protein CTSH in vitro and in vivo.Overexpression of CTSH in cells significantly increased the genome copy number and protein expression level of APPV.On the contrary,af-ter knocking down CTSH with siRNA,APPV proliferation showed a significant inhibitory effect.In sum-mary,CTSH interacts with Mut and promotes APPV replication.The results not only help to understand the interaction mechanism between virus and host cells,but also provide new ideas and methods for the prevention and treatment of viral diseases such as atypical plague.