闽北地区鸡毒支原体和滑液囊支原体多位点序列分型与毒力基因分析OA

To investigate the population structure,evolutionary relationships,and carriage of virulence genes of My-coplasma gallisepticum(MG)and Mycoplasma synoviae(MS)in the Fujian Minbei region,we used six MG housekeeping genes(atpG,dnaA,fasA,rpoB,ruvB,uvrA)and seven MS housekeeping genes(Adk,atpG,Efp,Gmk,nagC,Ppa,recA)from the PubMLST database as typing references.We performed sequencing and multilo-cus sequence typing(MLST)on 30 MG nucleic acid-positive samples and 41 MS nucleic acid-positive samples col-lected from large-scale chicken farms in Minbei between February and October 2023.The evolutionary relationships among different strains were analyzed.The results showed that among the 6 MG-positive samples,all 6 housekeep-ing genes were successfully amplified and sequenced,and they were classified into 3 sequence types(STs):ST78,ST130,and ST133.The predominant ST was ST78(50％),and according to evolutionary relationships,they were categorized into A-D4 groups,with the predominant strain being closely related to the vaccine strain.Among the 10 MS-positive samples,all 7 housekeeping genes were successfully amplified and sequenced,and they were classified into 6 STs:ST43,ST80,ST93,ST96,ST147,and ST151.The predominant ST was ST147(30％),and according to evolutionary relationships,they were categorized into A-E5 groups,with only 2 ST43 strains co-occurring with MS-H strains in group A.The virulence gene prediction results indicated that MG in the Minbei region carried 10 viru-lence genes,including tuf,pdhB,vlh,and gap A,with plp A as the main mediator.MS carried 7 virulence genes,including tuf,plr,gapA,and pdhB,with tuf and tnp as the main mediators.This study enriches the epidemiolog-ical data of mycoplasma in large-scale chicken farms in the Fujian Minbei region and provides a reference for the prevention,control,and eradication of mycoplasma infections in chickens.