三七总皂苷对大鼠放射性心脏损伤的保护作用及机制研究OA

Objective:To investigate the protective effect of Panax Notoginseng Saponins(PNS)on radiation-induced heart disease(RIHD)in rats and explore its underlying mechanism.Methods:A total of 50 rats were randomly divided into 5 groups using a random number table:normal control group,radiation-only group,and low-,medium-,and high-dose PNS intervention groups,with 10 rats in each group.Except for the normal control group,rats in the other four groups received a single 20 Gy cardiac irradiation.Rats in the low-,medium-,and high-dose PNS groups were intragastrically administered with 10 mg/kg,30 mg/kg,and 60 mg/kg PNS,respectively,while those in the normal control and radiation-only groups received an equal volume of normal saline.The administration was initiated on the day of irradiation and continued daily for 4 weeks after radiotherapy.After the irradiation,serum levels of cardiac troponin I(cTnI),creatine kinase isoenzyme MB(CK-MB),malondialdehyde(MDA),superoxide dismutase(SOD),intercellular adhesion molecule-1(ICAM-1),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and transforming growth factor-β1(TGF-β1)were detected.Pathological changes of myocardial tissues were observed using hematoxylin-eosin(HE)staining.Western blot was performed to determine the protein expression levels of TGF-β1 and Smad3 in myocardial tissues.Results:Compared with the normal control group,the radiation-only group showed increased serum levels of cTnI,CK-MB,MDA,ICAM-1,TNF-α,IL-1β,and TGF-β1(P<0.05),decreased serum SOD level(P<0.05),and elevated myocardial pathological score(P<0.05).Compared with the radiation-only group,all PNS intervention groups exhibited reduced serum levels of cTnI,CK-MB,MDA,ICAM-1,TNF-α,IL-1β,and TGF-β1(P<0.05),increased serum SOD level(P<0.05),and decreased myocardial pathological score(P<0.05).The changes of these indexes were dose-dependent in the low,medium and high dose groups(P<0.05).Conclusion:PNS exerts a protective effect against RIHD in rats,and its mechanism may be associated with regulating oxidative stress,alleviating inflammatory response,and inhibiting the TGF-β1/Smad3 signaling pathway.