木芙蓉种间杂交后代的真实性鉴定OA

Taking Hibiscus mutabilis'Danbanhong'and'Zuiyun'varieties as female parents and crossed them with Bombycidendron grewiifolium and H.moscheutos as male parents.A total of 72 progeny were obtained.Transcriptome sequencing of parental leaves was conducted to identify divergent SSR loci,and corresponding primers were designed for amplification and screening,in order to provide reference for the identification of interspecific hybridization of orther plants.The results showed that eight pairs of primers exhibited polymorphisms between the parents.In the DG combination,only SSR020 could authenticate the progeny,confirming DG-26 as a true hybrid.In the DM and ZM combinations,four pairs of primers were effective for progeny identification.Among the eight progeny in the DM combination,only DM-16 could not be definitively identified as a true hybrid,while the remaining seven were confirmed as true hybrids.In the ZM combination,SSR020 identified six out of 24 progeny as true hybrids.Phenotypic cross-validation demonstrated that the identified hybrids exhibited intermediate morphological traits between the parents,confirming the reliability and effectiveness of the molecular markers developed in this study.These markers provided valuable tools for interspecific hybridization between H.mutabilis and H.moscheutos,significantly shortening the breeding process.