基于拮抗和多基因序列分析平菇种质资源遗传多样性OA

[Objective]This study aimed to reveal the genetic variation and phylogenetic relationships of the tested Pleurotus ostreatus strains by combining cross-trial analysis with multi-gene sequence analysis techniques.[Method]Using 15 strains of P.ostreatus as test samples,the antagonistic interactions among strains were first determined through antagonism assays,and then the mycelial growth characteristics of the test strains on PDA plates were observed.Concurrently,genomic DNA was extracted from the test strains,and multiple gene se-quences were amplified to construct a phylogenetic tree.[Result]Mycelial growth characteristics revealed that the growth rates of Jinxiu-2 and Jinxiu-1 exhibited extremely significant differences compared to other strains,reach-ing to 4.722 mm·d⁻1.Xiangxiu-1 showed the slowest growth rate at 2.361 mm·d⁻1.Antagonistic interactions ex-isted to varying degrees among most strains,with only Jinxiu-2 and Jinxiu-1 exhibiting no antagonistic response.The total GC content in the ITS sequences ranged from 43.44%to 46.71%,with genetic distances varying from 0.002 to 0.397.The total GC content in the TEFI-α sequences ranged from 52.06%to 55.06%,with genetic dis-tances ranging from 0.021 to 0.445.Based on the ITS+TEFI-α phylogenetic tree,antagonistic response,and growth rate results,test strains C,D,F,G,N,and P formed one group,strains A,E,H,I,J,L,and M formed an-other group,and strain B stood alone as a separate group.[Conclusion]This study combined antagonism assays with polygenic sequence analysis to demonstrate that P.ostreatus germplasm resources exhibit substantial genetic diversity,with significant genetic differences among strains.These findings provide concrete evidence for the classification,identification,and utilization of P.ostreatus germplasm resources.