环境变化对连翘甾体和鞣质代谢的影响OA

[Objective]Steroids and tannins metabolites play a key role in the pharmacological effects of For-sythia suspensa.This study investigated the impacts of environmental factors on the metabolism of steroids and tan-nins in F.suspensa from a population perspective,aiming to provide theoretical support for addressing environmental changes and ensuring the high-quality and stable supply of F.suspensa medicinal materials.[Method]The relative contents of 19 steroids and tannins metabolites were analyzed in 10 populations of F.suspensa using ultra perfor-mance liquid chromatography and tandem mass spectrometry(UPLC-MS/MS),and then the data were intergrated with environmental factors to explore the impact of environmental changes on F.suspensa metabolites.[Result]Sig-nificant differences were oberved in 12 metabolites among different populations.The populations of F.suspensa from Huixian county(HX)and Lushi county(LS)of Henan Province exhibited higher contents of steroids and tan-nins,suggesting these areas as priority for cultivation.In terms of environmental factors,six metabolites(petasiphe-nol,phyllanemblinin D,abutasterone,corilagin,chestanin,casuarinin)were significantly affected by temperature,while two metabolites(5 α-pregnane-3,20-dione,praecoxin A)were significantly affected by precipitation.[Con-clusion]This study confirmed that environmental changes have significant impacts on the levels of steroids and tan-nins metabolites in F.suspensa.Different metabolites respond differently to hydrothermal conditions;therefore,suitable environmental conditions should be selected based on the desired metabolites during cultivation.