盐驯化通过调节光合作用和抗氧化系统提高大豆幼苗耐盐性OA
Salt acclimation improves salt tolerance of soybean seedlings by regulating photosynthesis and antioxidant systems
[目的]探明盐驯化对大豆耐盐性的影响,揭示盐驯化影响大豆耐盐性的生理机制.[方法]以'苏黄 1号'大豆为试验材料,经过 80 mmol·L-1 NaCl 盐驯化处理 24 h后,再转移到不同浓度的盐处理液(0、120、160和200 mmol·L-1 NaCl)中进行培养,研究盐驯化对盐胁迫下大豆幼苗生长、光合特性和抗氧化系统的影响.[结果]盐驯化组大豆幼苗的株高和地上部鲜质量随盐处理浓度的升高而升高,对照组的株高和地上部鲜质量随盐处理浓度的升高而降低.盐驯化组叶片的叶绿素指数、类黄酮指数和多光谱荧光参数随盐处理浓度的升高呈先升高后降低的变化趋势,对照组的叶绿素指数和氮平衡指数随盐处理浓度的升高而呈逐渐降低的趋势.盐驯化组的最大光合效率在 200 mmol·L-1 盐处理下显著降低(降低了 24.17%),对照组的最大光合效率在 160和 200 mmol·L-1 盐处理下均显著降低(分别降低了 41.49%和 30.13%).盐驯化组的净光合速率、蒸腾速率、胞间 CO2浓度和气孔导度在各浓度盐处理之间无显著变化,而对照组的上述光合参数随盐处理浓度升高而降低;在同浓度的盐处理下,盐驯化组的上述光合参数均显著高于对照组.盐驯化组在盐胁迫下的SOD和POD活性均比0 mmol·L-1 NaCl条件下显著提高.[结论]盐驯化后大豆幼苗在盐胁迫下可维持良好的生长状态和较稳定的光合特性,且抗氧化酶活性增强并缓解了盐胁迫造成的氧化损伤,最终提高了大豆幼苗的耐盐性.
[Objective]This study aimed to investigate the effect of salt acclimation on soybean salt tolerance,elucidate the physiological mechanisms underlying salt acclimation-enhanced salt tolerance in soybean.[Method]After being subjected to 80 mmol·L-1 salt acclimation treatment for 24 h,the plants were then transferred to different salt treatment solutions(0,120,160 and 200 mmol·L-1 NaCl)for cultivation.The effect of salt stress on plant height,photosynthetic properties and antioxidant system of soybean seedlings were investigated.[Result]The plant height and above-ground fresh weight of the control group decreased as the salt treatment concentration increased.The chlo-rophyll index of the leaves in the salt-acclimation group decreased.The chlorophyll index,flavonoid index and multi-color fluorescence parameters of leaves in the salt-acclimation group showed a trend of first increasing and then de-creasing with the increase of salt treatment concentration.In contrast,the chlorophyll index and nitrogen balance index of the control group showed a gradually decreasing trend with the increase of salt treatment concentration.The maxi-mum photosynthetic efficiency of salt-acclimation group did not differ significantly between 120 and 160 mmol·L-1 salt treatment and significantly decreased by 24.17%under 200 mmol·L-1 salt treatment.The maximum photosyn-thetic efficiency of the control group was significantly decreased under 160 and 200 mmol·L-1 salt treatment(de-creasing by 41.49%and 30.13%,respectively).The net photosynthetic rate,transpiration rate,intercellular CO2 con-centration and stomatal conductivity of the salt-acclimation group did not change significantly.Meanwhile,the above-mentioned photosynthetic parameters of the control group decreased with the increase of the salt treatment concentration.Under the same concentration of salt treatment,the above photosynthetic parameters of the salt-acclimation group were significantly higher than that of the control group.The SOD and POD activities of the salt-acclimation group under salt stress were significantly higher than those under 0 mmol·L-1 NaCl conditions.[Conclusion]After salt acclimation,soybean seedlings can maintain a good growth state and relatively stable pho-tosynthetic characteristics under salt stress.The antioxidant enzyme activities enhanced and alleviated the oxidative damage caused by salt stress,ultimately improving the salt tolerance of soybean seedlings.
周梦杰;尚辉;张晓燕;纪晓伟;吴谷丰;黄璐;陈新;薛晨晨
江苏省沿海开发(东台)有限公司,江苏 盐城 224237江苏省沿海开发(东台)有限公司,江苏 盐城 224237江苏省农业科学院经济作物研究所,江苏 南京 210014江苏省农业科学院经济作物研究所,江苏 南京 210014江苏省农业科学院经济作物研究所,江苏 南京 210014||江苏大学食品与生物工程学院,江苏 镇江 212013江苏省农业科学院经济作物研究所,江苏 南京 210014江苏省农业科学院经济作物研究所,江苏 南京 210014||江苏大学食品与生物工程学院,江苏 镇江 212013江苏省农业科学院经济作物研究所,江苏 南京 210014||江苏大学食品与生物工程学院,江苏 镇江 212013
农业科技
大豆盐驯化耐盐性多光谱荧光成像抗氧化活性
soybeanssalt acclimationsalt resistancemulticolor fluorescence imagingantioxidant activity
《安徽农业大学学报》 2026 (2)
207-215,9
江苏省沿海开发集团青年创新工作室项目(2023YHDTQC06)江苏省种业振兴揭榜挂帅项目(JBGS-2021-014)
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