4个种源长白落叶松抗虫性差异的初步评价OA

[Objective]Using four provenances of Larix olgensis(Tianqiaoling,Baihe,Dahailin,Baidaoshan)as experimental materials,this study aimed to evaluate the differences in resistance to the larch caterpillar(Dendro-limus superans)among them.[Method]Detached branches were collected for a water-culture experiment combined with a feeding trial using D.superans,thirteen insect resistance-related parameters were systematically measured,including three phenotypic traits(heavy feces,insect weight,defoliation rate)and ten physiological indices(activities of defense enzymes:SOD,POD,CAT,PPO,PAL;contents of secondary metabolites:tannins,total phenolics,fla-vonoids;and activities of protease inhibitors:TI,CI).[Result]There were significant or highly significant differ-ences(P<0.05 or P<0.01)among the provenances in both phenotypic traits of insect resistance(larval weight,fecal weight,leaf loss rate)and physiological indicators.Larvae fed on the Baihe provenance produced higher fecal weight and attained higher larval weight compared to those fed on other provenances.The Baihe provenance exhibited higher CAT and PPO activities than those of the other provenances.Conversely,the TI and CI contents in the Baihe provenance were lower than in the others.Tianqiaoling provenance had higher contents of secondary metabolites than the other provenances.[Conclusion]A comprehensive evaluation using the membership function method based on the three post-feeding phenotypic indicators and ten resistance-related physiological indicators revealed the following ranking of insect resistance for the four provenances:Baihe>Baidaoshan>Dahailin>Tianqiaoling.The hydroponic experiment provides a preliminary evaluation of the insect resistance of different L.olgensis provenances,offering a theoretical basis for screening insect-resistant germplasm resources.However,field trials are still required for further validation to gain deeper insights into the insect resistance of the tested materials.