瑞鲍迪苷M的急性经口毒性和遗传毒性检测OA

OBJECTIVE:To detect acute oral toxicity and genotoxicity of rebaudioside M,a new food additive,and to evaluate its health risks.METHODS:For the acute oral toxicity test,20 Kunming mice and 20 SD rats(half male and half female)were selected,and administered by gavage at a dose of 10 g/kg to estimate the median lethal dose(LD50)of rebaudioside M.A battery of genotoxicity tests was performed,including the mammalian erythrocyte micronucleus test,in vitro mammalian chromosome aberration test,and bacterial reverse mutation test,in accordance with national food safety standards.The dosages used in the micronucleus test were 1 250,2 500,and 5 000 mg/kg;those in the in vitro mammalian chromosome aberration test were 1.25,2.50,and 5.00 mg/mL;and those in the bacterial reverse mutation test were 0.008,0.04,0.20,1.00,and 5.00 mg per plate.RESULTS:The acute oral LD50 of the test substance in both KM mice and SD rats was greater than 10 g/kg body weight.Compared with the negative control group,there were no significant differences in micronucleated cell rates in all dose groups.No significant differences in chromosome aberration rates were observed in all dose groups either with or without S9 metabolic activation.The number of revertant colonies in all dose groups did not exceed twice that of the solvent control group.CONCLUSION:Under the conditions of this study,rebaudioside M can be classified as non-toxic with no genotoxicity.