基于非靶向代谢组学的甘肃不同地区浆水代谢物差异分析OA

[Objective]This study aimed to investigate the metabolomic differences among Jiang-shui samples from different regions of Gansu Province.[Methods]Jiang-shui samples collected from Dingxi,Lanzhou,and Tianshui in Gansu Province were subjected to non-targeted metabolomics analysis using ultra-high performance liquid chromatography coupled with Orbitrap ExplorisTM 480 high-resolution mass spectrometry(UHPLC-Orbitrap ExplorisTM 480 MS)combined with multivariate statistical analysis.[Results]A total of 1746 metabolites were detected in the Jiang-shui samples from the three regions,with 1107 and 639 metabolites identified in positive and negative ion modes,respectively.These included 219 lipids and lipid-like molecules,96 phenylpropanoids and polyketides,446 organic heterocyclic compounds,360 organic acids and derivatives,284 benzenoids,183 organic oxygen compounds,49 organic nitrogen compounds,and 5 organic sulfur compounds.The Tianshui samples showed significant differences from those of the other two regions,whereas the Dingxi and Lanzhou samples exhibited certain similarities.Orthogonal partial least squares-discriminant analysis(OPLS-DA)with VIP>1 and P<0.05 identified 568 differential metabolites,including 36 taste-related metabolites.Among them,sour and bitter substances were the main contributors to the taste of Jiang-shui;sour substances were predominantly accumulated in Tianshui samples,while bitter substances were mainly concentrated in Lanzhou samples.Among the 21 bioactive substances identified,phenolic acids and organic acids were relatively abundant.Kyoto Encyclopedia of Genes and Genomes(KEGG)annotation revealed that the alanine,aspartate and glutamate metabolism pathway was significantly enriched among the differential metabolites from the three regions.[Conclusion]The Jiang-shui samples from different regions exhibited significant differences in metabolite composition,abundance,and metabolic pathways.These findings provide a reference for further understanding the quality formation mechanism of Gansu Jiang-shui.