花叶饮代用茶对高脂血症大鼠降脂作用及肠道菌群调控研究OA

Objective:To investigate the lipid-lowering effect and intestinal flora modulation of Huayeyin herbal substitute tea in hyperlipidemic rats.Methods:SD rats were divided into blank group,model group,and low-,medium-and high-dose Huayeyin groups.The hyperlipidemia rat model was established by intragastric administration of high-fat and high-sugar diet com-bined with lard.After successful modeling,the rats were administered with corresponding interventions for 4 weeks.The serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cho-lesterol(HDL-C),as well as the activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)were detected.Liver tissues were stained with hematoxylin-eosin(HE)and Oil Red O.16S rRNA sequencing was performed to analyze the changes of intestinal flora.Results:Compared with the blank group,the weight growth rate,serum TC,TG,LDL-C levels and AST activity in the model group were significantly increased,while HDL-C level was significantly decreased.Obvious hepatic ste-atosis and inflammatory lesions were observed in liver tissues.The abundances of Firmicutes,Lactobacillus and Prevotella were decreased,whereas the abundances of Proteobacteria and Anaerospirillum were increased(all P<0.05 or P<0.01).Compared with the model group,the weight growth rate in the high-dose Huayeyin group was significantly reduced in the first week;the levels of TC,TG and LDL-C and the activity of AST were significantly decreased in each dose group,and ALT activity was significantly reduced in the medium-dose group,with alle-viated hepatic steatosis and inflammation.The abundances of Firmicutes and Blautia were significantly upregulated in all dose groups;the abundances of Proteobacteria and Anaerospi-rillum were markedly downregulated in the high-dose group,and the abundance of Prevotella was significantly elevated in the medium-dose group(all P<0.05 or P<0.01).Correlation analysis showed that the abundance of Lactobacillus was sig-nificantly positively correlated with HDL-C level(P<0.05).The abundances of Lactobacillus and Prevotella showed a negative correlation trend with TC,TG and LDL-C levels and AST and ALT activities,and a positive correlation trend with HDL-C level;while Anaerospirillum presented the opposite trend.Conclusion:Huayeyin herbal substitute tea lowers serum lipids,ameliorates liver pathology,and regulates gut dysbiosis in hyper-lipidemic rats.The results support its use in daily prevention and adjunctive treatment of hyperlipidemia.