诱导三阴性乳腺癌细胞凋亡的Miliusol衍生物的发现及其体内外抗TNBC活性评价OA

Objective:To synthesize and explore the role of Miliusol derivatives in inducing apoptosis in triple-negative breast cancer cells and their effects on tumor activity.Methods:Through structural modification of the naturally occurring small molecule Miliusol,a novel derivative designated as YH8 was synthesized.The MTT assay,colony formation assay,transwell as-say,and scratch assay were used to evaluate the effects of Miliusol(0.8 μM)and its derivative YH8(0.4,0.8 μM)on the prolifera-tion and migration capacity of 4T1 cells.Flow cytometry and western blot analysis were employed to assess the apoptotic effects of Miliusol and YH8 on 4T1 cells.The in vivo antitumor effect of YH8(10 mg·kg-1)was evaluated using a 4T1 tumor-bearing mouse model.Results:Both Miliusol and YH8 inhibited the proliferation and migration of 4T1 cells in vitro and induced apopto-sis.Under equal dosage conditions,YH8 demonstrated superior efficacy compared to Miliusol.Furthermore,YH8 significantly in-hibited the growth of 4T1 tumors in vivo.Conclusion:YH8 effectively inhibits the growth of triple-negative breast cancer cells both in vitro and in vivo,with its primary mechanism of action being the induction of apoptosis.