三棱-莪术有效组分配伍改善黄褐斑的作用研究OA

Objective:To investigate the effect of the active component compatibility of Sparganii Rhizoma and Curcumae Rhizoma on improving chloasma.Methods:In vitro,the effects of total flavonoids from Sparganii Rhizoma and Curcumae Rhi-zoma oil on melanin production and tyrosinase activity in α-MSH-induced B16 cells were evaluated to determine the optimal com-patibility ratio.In vivo,a chloasma model was established on the auricles of C57BL/6 mice by progesterone injection combined with ultraviolet B irradiation.Serum samples were collected to detect superoxide dismutase(SOD)activity and malondialdehyde(MDA)content.Histopathological changes and melanin distribution in the auricular tissue were observed using hematoxylin-eosin(H&E)staining and Masson Fontana staining,respectively.Results:In vitro experiments showed that,compared with the control group,tyrosinase activity and melanin content were significantly increased in the model group(P<0.01).The compatibility of total flavonoids from Sparganii Rhizoma and Curcumae Rhizoma oil(1:1)effectively inhibited this increasing trend(P<0.01),with the most significant effect observed at this ratio and in a concentration-dependent manner.In vivo,compared with the normal group,the model group exhibited significantly decreased serum SOD activity(P<0.01),increased MDA level(P<0.05),and enhanced melanin distribution in the auricular tissue(P<0.001).Compared with the model group,treatment with the 1:1 compatibility of to-tal flavonoids from Sparganii Rhizoma and Curcumae Rhizoma oil improved melanin distribution in the auricular tissues of mice in all treatment groups(P<0.001),with the high-dose group significantly upregulating SOD activity(P<0.05)and downregulating MDA level(P<0.05).Conclusion:The compatibility of total flavonoids from Sparganii Rhizoma and Curcumae Rhizoma oil(1:1)has shown an ameliorative effect on chloasma,and its mechanism may be related to inhibiting melanin production and enhanc-ing antioxidant capacity.