TFAP2A调控支气管肺发育不良相关基因ACSL1的作用机制OA

Objective Expl ore the mechanism by which the transcription factor TFAP2A regulates gene ACSL1 in bronchopulmonary dysplasia.Methods Analysis of the relative RNA expression levels of BDP in umbilical cord blood of low gestational age preterm infants using the GSE8586 Affymetrix Human Genome U133 Plus 2.0 Array gene chip dataset;The possible binding sites of transcription factor TFAP2A in the promoter region of AC-SL1 in the JASPAR online database was searched;Overexpress and knockdown TFAP2A were performed in BE-AS-2 B cells;TFAP2A binding and activation of ACSL1 mRNA transcription were verified through ChIP and dual luciferase reporter gene experiments.The expression levels of TFAP2A,ACSL1 mRNA and protein were deter-mined using RT-qPCR and WB.Results In the GDS3356 data of BDP patients,it was found that the mRNA ex-pression levels of ACSL1 and TFAP2A significantly increased;the JASPAR online database revealed that the transcription factor TFAP2A had binding sites in the promoter region of ACSL1.The dual-luciferase reporter gene assay showed that overexpression of TFAP2A significantly increased the luciferase activity of the ACSL1 promot-er,and this increase was concentration-dependent;conversely,it was decreased.Overexpression of TFAP2A led to an increase in the mRNA and protein expression levels of both TFAP2A and ACSL1.Small interfering TFAP2A resulted in a decrease in the mRNA and protein expression levels of TFAP2A,TFAP2A,and ACSL1.Conclusion The promoter region of ACSL1 can specifically bind to TFAP2A.TFAP2A positively regulates the ex-pression of the ACSL1 gene,thereby increasing the number of transcription factor members that regulate important genes for bronchopulmonary dysplasia.