复方茵丹汤调节肠道菌群改善急性肝内胆汁淤积OA

Objective To investigate the effect of Compound Yindan Decoction(CYD)on the structure and composition of intestinal flora in rats with acute intrahepatic cholestasis(AIC)based on 16S rRNA sequencing.Methods Forty SD rats were randomly divided into normal group,model group,high,medium and low dose CYD(24.48,12.24,6.12 g·kg-1·d-1)groups,with 8 rats in each group.Except for the normal group,the other groups were given α-naphthylisothiocyanate(ANIT,60 mg/kg)to induce AIC model on the third day of the experiment,and each administration group was given CYD for 5 days before and after modeling.The intestinal flora was analyzed by 16S rRNA sequencing.Results The α diversity analysis showed that the species diversity of the middle and low dose CYD groups was lower than that of the model group(P＜0.05).In the analysis of β diversity,principal coordinate analysis(PCoA)suggested that the species composition of the high and low dose CYD groups was significantly different;unweighted pair group method with arithmetic mean(UPGMA)clustering suggested that the flora structure of the high dose CYD group of was close to the normal group.Anosim and multirespons permutation procedure(MRPP)analysis showed that there was a significant difference in the structure of the flora between the high and low dose CYD groups(P＜0.05).Differential species analysis showed that compared with the model group,the relative abundance of Bacteroidetes in the high dose CYD group increased at the phylum level,and the Firmicutes in the high and low dose CYD groups decreased(P＜0.01,P＜0.05).At the family level,the abundance of Prevotaceae in the high and low dose CYD groups increased(P＜0.05),and the grace of Lactobacillaceae,Lachnospiraceae,Oscillospiraceae and Ruminococcaceae in the low dose CYD group decreased(P＜0.01,P＜0.05).At the genus level,the abundance of Prevotellaceae-related bacteria in the high dose CYD group increased(P＜0.01),and the abundance of Lactobacillus and Ruminococcus decreased(P＜0.05).In the low dose CYD group,Lactobacillus and Ruminococcus decreased,and Prevotellaceae-related bacteria increased(P＜0.05).Linear discriminant analysis effect size(LEfSe)analysis showed that the characteristics of Proteobacteria,Prevotaceae and Proteobacteria were obvious in the low dose CYD group,and the characteristics of Bacteroidetes,families and orders were obvious in the middle dose CYD group.Conclusion CYD can improve ANIT-induced cholestasis in AIC rats,and its mechanism may be related to the regulation of intestinal flora structure(such as Bacteroidetes,Firmicutes,Prevotaceae,etc.),repair of intestinal barrier,and reduction of endotoxin and inflammatory factor release.