平肝透邪方治疗失眠伴中医烦躁焦虑状态的临床观察与网络药理学及分子对接研究OA
Clinical observation and network pharmacology and molecular docking study on the treatment of insomnia accompanied by irritation and anxiety state in traditional Chinese medicine by Pinggantouxie formula
目的 观察平肝透邪方对失眠伴中医烦躁焦虑状态患者的临床疗效,并利用网络药理学、分子对接技术分析机制.方法 选取2024年6月至2025年4月于日照市中医医院治疗的60例失眠伴中医烦躁焦虑状态患者为研究对象,给予平肝透邪方治疗4周.比较治疗前后患者的血常规、肝功能和肾功能差异评估安全性;比较治疗前后匹兹堡睡眠质量指数量表(Pittsburgh sleep quality index,PSQI)、汉密尔顿焦虑量表(Hamilton anxiety scale,HAMA)、中医证候积分差异评价有效性.利用网络药理学、分子对接技术分析平肝透邪方治疗失眠的机制.结果 与治疗前比较,治疗后患者的血常规、肝功能、肾功能差异无统计学意义(P>0.05);治疗后患者的PSQI、HAMA、中医证候积分降低,差异有统计学意义(P<0.05).网络药理学结果显示平肝透邪方治疗失眠的关键靶点有白蛋白、蛋白激酶B(protein kinase B,PKB,即AKT1)、白细胞介素(interleukin,IL)-6、肿瘤坏死因子、IL-1β,关键成分为槲皮素、茉莉酮、4-(4'-羟基苄氧基)苄基甲基醚、苏齐内酯、N-反式阿魏酸酪胺,关键通路包括神经活性配体-受体相互作用、神经退行性病变信号通路-多种疾病、环磷酸腺苷信号通路、辅助性T细胞17细胞分化等.分子对接结果显示关键靶点与成分均结合紧密,苏齐内酯、槲皮素与AKT1结合活性最强.结论 平肝透邪方可治疗失眠伴中医烦躁焦虑状态,通过多成分-多靶点-多通路发挥作用.
Objective To observe the clinical efficacy of Pinggantouxie formula in patients with insomnia accompanied by irritation and anxiety state in traditional Chinese medicine(TCM),and analyze its mechanism by using network pharmacology and molecular docking.Methods 60 patients with insomnia accompanied by irritation and anxiety state in TCM admitted to Rizhao Hospital of Traditional Chinese Medicine from June 2024 to April 2025 were selected as the research subjects,and were treated with Pinggantouxie formula for 4 weeks.Compare the changes in blood routine,liver function,and kidney function of patients before and after treatment to evaluate safety,and compare the changes in scores of Pittsburgh sleep quality index(PSQI),Hamilton anxiety scale(HAMA),and TCM syndrome scale before and after treatment to evaluate effectiveness.Results Compared with before treatment,there were no statistically significant differences in blood routine,liver function and renal function after treatment(P>0.05).After treatment,the scores of PSQI,HAMA and TCM syndrome scale were significantly decreased,with statistically significant differences(P<0.05).Network pharmacology results showed that the key targets of Pinggantouxie formula in the treatment of insomnia included albumin,protein kinase B(PKB,namely AKT1),interleukin(IL)-6,tumor necrosis factor and IL-1β.The key active ingredients were quercetin,jasmone,4-(4'-hydroxybenzyloxy)benzyl methyl ether,suzilactone and N-trans-feruloyltyramine.The key signaling pathways mainly involved neuroactive ligand-receptor interaction,multiple neurodegenerative disease pathways,cyclic adenosine monophosphate signaling pathway,and T helper 17 cell differentiation pathway.Molecular docking results indicated that the key ingredients bound closely to the core targets.Among them,suzilactone and quercetin exhibited the strongest binding activity with AKT1.Conclusion Pinggantouxie formula can effectively treat insomnia accompanied by TCM irritability and anxiety state,and exerts its therapeutic effects through a multi-component,multi-target and multi-pathway mechanism.
刘呈祥;宫庆东;徐星华;李艳君;刘雪梅;牟磊
日照市中医医院脑病科,山东 日照 276800日照市中医医院脑病科,山东 日照 276800日照市中医医院脑病科,山东 日照 276800日照市中医医院心血管病科,山东 日照 276800日照市中医医院心血管病科,山东 日照 276800日照市中医医院脑病科,山东 日照 276800
医药卫生
失眠中医烦躁焦虑状态平肝透邪方网络药理学
InsomniaIrritation and anxiety state in TCMPinggantouxie formulaNetwork pharmacology
《中国现代医生》 2026 (15)
48-53,79,7
山东省日照市中医药科技项目(RZY2023C03)
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