湖北恩施马铃薯病毒病流行与分布特征OA

To clarify the types and occurrence of viruses in the main potato production areas of Enshi,virus detection was conducted on 430 self-retained potatoes from farmers using double antibody sandwich-enzyme linked immunosorbent assay(DAS-ELISA),next-generation sequencing(NGS)and reverse transcription-polymerase chain reaction(RT-PCR).The results showed that potato virus S(PVS)and potato virus M(PVM)were the main pathogens causing potato virus diseases in this region.The detection rates of PVS and PVM were 53.5％and 23.0％respectively,and the detection rates of other virus species were all below 10.0％.The detection rate of viruses varies with changes in altitude.Potato virus Y(PVY)had the highest detection rate of 8.2％in low-altitude areas(＜700 m),potato virus X(PVX)had the highest detection rate of 12.3％in mid-altitude areas(700-1 000 m),PVS and PVM had detection rates as high as 64.2％and 33.0％,respectively,in high-altitude areas(＞1 000 m).Potato leafroll virus(PLRV)and potato virus A(PVA)showed no significant difference in distribution across altitudes.The epidemiological patterns of potato viruses vary from region to region.Badong and Jianshi were most severely infected by PVS and PVM,with PVS detection rates of 90.6％and 69.8％,respectively,and PVM detection rates of 58.8％and 34.0％,respectively.PVX and PVA were more prevalent in Hefeng,with detection rates of 20.0％and 35.0％,respectively.The main patterns of potato virus infection in Enshi were single and double infections,accounting for 52.8％and 37.8％of the infected samples,respectively,while triple and quadruple infections occurred less frequently,accounting for only 8.7％and 0.7％,respectively.Additionally,mild infections of potato virus H(PVH)and potato mop-top virus(PMTV)were present in some areas of Enshi.This study provides important basic information for prevention and control of potato viruses in the Enshi region.