水稻SPX基因家族鉴定及响应磷处理的表达分析OA
Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment
本研究旨在全基因组水平系统鉴定水稻 SPX 基因家族成员,分析其进化特征与表达模式,为阐明该家族在磷信号转导中的功能提供理论依据.利用生物信息学方法对水稻SPX基因家族进行全基因组鉴定,分析其理化性质、系统进化关系、基因结构、保守基序、启动子顺式作用元件及共线性关系;基于转录组数据和实时荧光定量 PCR(RT-qPCR)技术解析其组织表达特性及磷胁迫应答模式.本研究共鉴定到 6 个水稻SPX基因,分布于 5 条染色体上.系统进化分析表明,OsSPX1 和OsSPX2 以 100%支持率聚类,OsSPX3 与OsSPX5/OsSPX6 形成稳定亚簇.共线性分析发现,有 2 对片段复制基因(OsSPX1/OsSPX2 和OsSPX5/OsSPX6),且Ka/Ks值均小于 1(0.19～0.44),表明该家族在进化过程中受强烈纯化选择.基因结构分析显示,OsSPX成员含 2～3 个外显子,保守基序分布模式与系统进化关系高度一致.启动子分析发现了 135 个顺式作用元件,主要包括激素响应、光响应和胁迫响应元件,其中茉莉酸甲酯和脱落酸响应元件最为丰富.基于RT-qPCR在ShijinB品种的表达模式分析表明,OsSPX1 至OsSPX6 在根中均表现出显著表达优势,具有普遍的根偏好性特征.此外,OsSPX3 在花序中也显示出较高的表达水平,而OsSPX1 和OsSPX4 在叶中的表达也较高.磷胁迫响应分析显示,OsSPX1、OsSPX2、OsSPX3、OsSPX5 和OsSPX6 在低磷条件下显著上调表达,其中,OsSPX3 表达量增长最为显著,而OsSPX4 表达不受磷浓度影响.水稻SPX基因家族通过片段复制扩张并受纯化选择作用,成员在基因结构和基序组成上较为保守,但在表达模式上呈现明显的组织特异性和磷胁迫应答分化.
This study aimed to systematically identify the rice SPX gene family at the whole-genome level,characterize its evolu-tionary features and expression patterns,and provide a basis for elucidating SPX functions in phosphorus signal transduction.Bioinformatics approaches were used to identify SPX genes in rice and to analyze their physicochemical properties,phylogenetic relationships,gene structures,conserved motifs,promoter cis-acting elements,and collinearity.Transcriptomic data and quantita-tive real-time PCR(RT-qPCR)were used to examine tissue-specific expression and responses to phosphorus stress.Six SPX genes were identified and mapped to five chromosomes.Phylogenetic analysis showed that OsSPX1 and OsSPX2 clustered together with 100%bootstrap support,whereas OsSPX3 formed a well-supported subclade with OsSPX5 and OsSPX6.Collinearity analy-sis identified two segmental duplication pairs(OsSPX1/OsSPX2 and OsSPX5/OsSPX6),and all Ka/Ks values were<1(0.19-0.44),indicating strong purifying selection.Gene structure analysis showed that OsSPX members contain 2-3 exons,and conserved motif distributions were highly consistent with the phylogenetic relationships.Promoter analysis identified 135 cis-acting ele-ments,mainly related to hormone,light,and stress responses,with methyl jasmonate-and abscisic acid-responsive elements being the most abundant.RT-qPCR analysis in the ShijinB variety indicated that OsSPX1-OsSPX6 were preferentially expressed in roots,with OsSPX3 also showing relatively high expression in inflorescences and OsSPX1 and OsSPX4 showing relatively high expression in leaves.Under low-phosphorus conditions,OsSPX1,OsSPX2,OsSPX3,OsSPX5,and OsSPX6 were significantly upregulated,with OsSPX3 showing the strongest induction,whereas OsSPX4 was not affected by phosphorus availability.Overall,the rice SPX gene family appears to have expanded via segmental duplication and subsequently evolved under purifying selection;although gene structures and motif compositions are relatively conserved,members show clear tissue specificity and differential responsiveness to phosphorus stress.
胡赵;钱润;谢丰璞;应素平
太原师范学院生物科学与技术学院,山西 晋中 030619||南昌大学生命科学学院,江西 南昌 330031沈阳药科大学中药学院,辽宁 沈阳 110016沈阳药科大学中药学院,辽宁 沈阳 110016南昌大学生命科学学院,江西 南昌 330031
水稻SPX基因家族磷胁迫生物信息学表达分析
riceSPX gene familyphosphorus stressbioinformaticsexpression analysis
《作物学报》 2026 (6)
1902-1912,11
本研究由山西省基础研究计划项目(202503021212246)资助.This study was supported by the Fundamental Research Program of Shanxi Province(202503021212246).
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