水培盐碱复合胁迫下'济麦60'苗期根系有机酸分泌动态变化及其转录调控机制OA
Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in'Jimai 60'seedlings under combined salinity-alkalinity stress in hydroponics
土壤盐碱化是全球农业生产面临的主要限制因素之一,显著制约小麦产量并威胁粮食安全.植物根系分泌有机酸是应对非生物胁迫的重要生理策略.然而,在盐碱复合胁迫下,小麦根系有机酸分泌的动态特征及其背后的转录调控网络尚缺乏系统研究.本研究以耐盐碱小麦品种'济麦 60'为材料,系统分析了其在盐碱复合胁迫不同时间点根系有机酸的代谢组与转录组变化特征,并结合加权基因共表达网络分析(WGCNA)挖掘关键调控模块.结果表明,盐碱复合胁迫显著改变了根系有机酸的组成与含量,在4个比较组中共鉴定到44种差异积累的有机酸,其中12种持续上调,4 种持续下调.转录组分析显示,随着胁迫时间的延长,差异表达基因数量逐步增加,与光合作用、有性生殖和卟啉代谢等相关的基因持续被激活,而细胞壁组织、氧化磷酸化等过程则表现出时间阶段性响应.WGCNA 分析进一步鉴定出MEblue、MEturquoise和MEyellow 3 个与关键有机酸代谢显著相关的功能模块,这些模块中的基因主要富集于能量代谢、氨基酸合成与氧化还原平衡等过程.本研究系统描绘了耐盐碱小麦品种'济麦 60'在盐碱复合胁迫下根系有机酸代谢与转录调控的动态图谱,这不仅加深了对小麦耐盐碱生理机制的认识,也为通过分子设计育种靶向改良根系功能与逆境适应性提供了重要的理论基础和基因资源.
Soil salinization is a major constraint on global agricultural production,substantially reducing wheat yield and threat-ening food security.Root secretion of organic acids is an important physiological strategy by which plants cope with abiotic stress.However,under combined salt-alkali stress,the temporal dynamics of organic acid secretion in wheat roots and the underlying transcriptional regulatory networks remain poorly understood.Here,the salt-alkali-tolerant wheat cultivar'Jimai 60'was used to systematically characterize time-resolved metabolomic and transcriptomic profiles of root organic acids under combined salt-alkali stress,and key regulatory modules were identified using WGCNA.Combined salt-alkali stress markedly reshaped the composition and abundance of root organic acids.Across four comparison groups,44 differentially accumulated organic acids were identified,including 12 that were consistently upregulated and 4 that were consistently downregulated.Transcriptome analy-sis showed that the number of differentially expressed genes increased progressively with longer stress exposure.Genes related to photosynthesis,sexual reproduction,and porphyrin metabolism were persistently activated,whereas cell wall organization and oxidative phosphorylation exhibited stage-specific responses.WGCNA further identified the MEblue,MEturquoise,and MEyel-low modules as significantly associated with major organic acid metabolic traits;genes in these modules were mainly enriched in pathways related to energy metabolism,amino acid biosynthesis,and redox homeostasis.Overall,this study delineates the dy-namic landscape of root organic acid metabolism and transcriptional regulation in salt-alkali-tolerant wheat('Jimai 60')under combined salt-alkali stress.These findings advance our understanding of the physiological basis of wheat salt-alkali tolerance and provide a foundation,as well as candidate gene resources,for molecular breeding aimed at improving root function and stress resilience.
陈雪燕;吕胜源;吴印莹;赵振东;曹新有;万何平;何华川;李政嘉;董新盼;李藕琪;刘小云;李丹萍;陈志伟;刘国霞
山东省农业科学院,山东 济南 250100山东省农业科学院,山东 济南 250100山东省农业科学院,山东 济南 250100山东省农业科学院,山东 济南 250100山东省农业科学院,山东 济南 250100江汉大学生命科学学院,湖北 武汉 430056江汉大学生命科学学院,湖北 武汉 430056江汉大学生命科学学院,湖北 武汉 430056江汉大学生命科学学院,湖北 武汉 430056江汉大学生命科学学院,湖北 武汉 430056江汉大学生命科学学院,湖北 武汉 430056山东省农业科学院,山东 济南 250100山东省农业科学院,山东 济南 250100山东省农业科学院,山东 济南 250100
小麦盐碱复合胁迫根系有机酸转录组加权基因共表达网络分析
wheatcombined saline-alkaline stressrootsorganic acidstranscriptomeWGCNA
《作物学报》 2026 (6)
1859-1875,17
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-03-06),山东省重点研发计划项目(2023LZGC009,2024LZGCQY001)和山东省泰山学者工程项目(tstp20240843)资助.This study was supported by the China Agriculture Research System of MOF and MARA(CARS-03-06),the Key Research and Development Program of Shandong Province(2023LZGC009,2024LZGCQY001),and the Taishan Scholar Program(tstp20240843).
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