小麦ABHD6基因家族鉴定与粒重功能标记开发OA
Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat
α/β-水解酶蛋白 6(α/β-hydrolasedomain-containing 6,ABHD6)作为丝氨酸水解酶超家族的重要成员,在调控植物生长发育及环境胁迫响应中发挥重要作用.然而,目前仍缺乏对小麦(Triticum aestivum L.)ABHD6 基因家族的系统性鉴定与分析.本研究综合利用系统进化、蛋白质序列分析、启动子顺式作用元件预测以及单倍型分析等方法,在小麦基因组中共鉴定出 18 个 TaABHD6 家族成员.系统进化与结构分析表明,小麦 TaABHD6 蛋白与水稻OsABHD6 蛋白亲缘关系较近,同一进化分支内的成员具有高度相似的保守结构域.启动子分析显示,TaABHD6 基因家族启动子区富含多种参与植物生长发育调控的顺式作用元件.通过分析 TaABHD6 基因家族成员的基因组多态性及其与籽粒表型的关联,鉴定出 8 个基因存在不同的单倍型.其中,3 个基因的不同单倍型与籽粒相关性状之间存在显著关联.TaABHD6-5 基因的不同单倍型与粒重、粒长、粒宽和粒厚均显著关联.针对TaABHD6-5 启动子区域-1045 bp处SNP(A/T)开发KASP分子标记,在305份小麦种质中进行验证,发现该功能标记可有效区分 2种单倍型,其中,TaABHD6-5-HapI单倍型品种 268 份,TaABHD6-5-HapII单倍型品种 34 份,杂合子 2 份,分型失败 1 份.籽粒表型数据关联分析表明,TaABHD6-5-HapI是增加粒重的优异单倍型.表达模式分析结果表明,TaABHD6-5 在孕穗期的茎、穗以及籽粒发育初期表达较高,且优异单倍型 TaABHD6-5-HapI 的表达量显著低于 TaABHD6-5-HapII,表明TaABHD6-5 可能为粒重负调控因子.基因序列分析表明,TaABHD6-5 受 miR160 靶向调控.本研究结果为阐明TaABHD6 基因调控小麦籽粒发育的生物学功能及其优异单倍型的应用价值提供重要理论支撑.
α/β-Hydrolase domain-containing protein 6(ABHD6),a key member of the serine hydrolase superfamily,plays impor-tant roles in regulating plant growth,development,and responses to environmental stress.However,a systematic genome-wide identification and analysis of the ABHD6 gene family in wheat(Triticum aestivum L.)is still lacking.Here,we integrated phylogenetic analysis,protein sequence characterization,promoter cis-acting element prediction,and haplotype analysis,and identified 18 TaABHD6 family members in the wheat genome.Phylogenetic and structural analyses showed that TaABHD6 pro-teins are closely related to rice OsABHD6 proteins,and that members within the same clade share highly conserved domains.Promoter analysis revealed that TaABHD6 promoters are enriched in cis-acting elements associated with plant growth and devel-opment.By analyzing polymorphisms in TaABHD6 genes and their associations with grain phenotypes,we identified eight genes with distinct haplotypes.Haplotype variation in three genes was significantly associated with grain-related traits;in particular,TaABHD6-5 haplotypes were significantly associated with grain weight,length,width,and thickness.We developed a KASP marker based on an A/T SNP at-1045 bp in the TaABHD6-5 promoter.Validation in 305 wheat accessions showed that this func-tional marker effectively distinguished two haplotypes:268 accessions carried TaABHD6-5-HapI,34 carried TaABHD6-5-HapII,two were heterozygous,and one accession failed genotyping.Association analysis further indicated that TaABHD6-5-HapI is a favorable haplotype for increased grain weight.qRT-PCR showed that TaABHD6-5 was relatively highly expressed in stems and spikes at booting and during early grain development;notably,expression was significantly lower in the favorable haplotype TaABHD6-5-HapI than in TaABHD6-5-HapII,suggesting that TaABHD6-5 may act as a negative regulator of grain weight.Se-quence analysis further suggested that TaABHD6-5 is targeted by miR160.Together,these results provide genome-wide evidence for the roles of TaABHD6 genes in wheat grain development and highlight the potential breeding value of superior haplotypes.
张献丰;郭利建;李康春;孔斌雪;刘玉芳;车卓;杨德龙
甘肃农业大学干旱生境作物学国家重点实验室,甘肃 兰州 730070||甘肃农业大学生命科学技术学院,甘肃 兰州 730070甘肃农业大学干旱生境作物学国家重点实验室,甘肃 兰州 730070甘肃农业大学干旱生境作物学国家重点实验室,甘肃 兰州 730070||甘肃农业大学生命科学技术学院,甘肃 兰州 730070甘肃农业大学农学院,甘肃 兰州 730070甘肃农业大学干旱生境作物学国家重点实验室,甘肃 兰州 730070||甘肃农业大学生命科学技术学院,甘肃 兰州 730070甘肃农业大学干旱生境作物学国家重点实验室,甘肃 兰州 730070甘肃农业大学干旱生境作物学国家重点实验室,甘肃 兰州 730070||甘肃农业大学生命科学技术学院,甘肃 兰州 730070||甘肃农业大学农学院,甘肃 兰州 730070
小麦α/β-水解酶结构域蛋白6(ABHD6)基因家族鉴定单倍型分子标记
wheatα/β-hydrolase domain-containing protein 6(ABHD6)gene family identificationhaplotypemolecular marker
《作物学报》 2026 (6)
1711-1727,17
本研究由甘肃省科技重大专项(25ZDNA001),甘肃省麦类产业技术体系项目(GSARS07),甘肃省教育厅青年博士支持项目(2024QB-062)和省部共建干旱生境作物学国家重点实验室开放基金项目(GSCS-2023-07)资助.This study was supported by the Gansu Provincial Science and Technology Major Project(25ZDNA001),the Gansu Province Wheat Industry Technology System Project(GSARS07),the Youth Doctoral Support Project of Gansu Provincial Department of Education(2024QB-062),and the Open Fund of the State Key Laboratory of Cropology in Arid Habitat Jointly Established by the Province and Ministry(GSCS-2023-07).
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