小麦籽粒铜含量的全基因组关联分析及候选基因预测OA
Genome-wide association study and candidate gene prediction of grain copper content in wheat
铜是人体必需的微量矿物质,缺乏铜会威胁人体健康.小麦为全球重要的口粮作物,借助生物强化提高小麦籽粒铜含量,是解决人体铜元素缺乏的最经济有效的措施.然而,目前小麦籽粒铜含量的遗传调控机制尚不清晰.本研究对 349 份小麦品系的籽粒铜含量进行测定,结合小麦 660K高密度SNP芯片进行全基因组关联分析(GWAS).结果显示,共检测到 775 个显著性SNPs,主要分布在 1B、4A和 7A染色体上,其中 56 个SNPs可以在 4 个及以上重复中被检测到;单倍型分析表明,GCuC_Hap_1B 和 GCuC_Hap_4A 可能是调控籽粒铜含量的重要基因位点,且二者具有明显的聚合效应;借助生物信息学分析和单倍型分析,推测TraesCS1B03G1265400 和TraesCS4A03G0093900 基因可能是调控小麦籽粒铜含量的候选基因.本研究解析了小麦籽粒铜含量的遗传机制,并为小麦品质改良育种中培育高籽粒铜含量种质资源提供重要的参考信息.
Copper is an essential trace element in humans,and copper deficiency can compromise health.Wheat is a major staple crop worldwide,and biofortification of wheat grain with copper is a cost-effective strategy to help alleviate dietary copper defi-ciency.However,the genetic basis of grain copper content in wheat remains poorly understood.Here grain copper content was evaluated in 349 wheat accessions,and a genome-wide association study(GWAS)was performed using the Wheat 660K SNP array.In total,775 significant SNPs were identified,mainly on chromosomes 1B,4A,and 7A;among these,56 SNPs were con-sistently detected in four or more replicates.Haplotype analysis indicated that GCuC_Hap_1B and GCuC_Hap_4A are key loci associated with grain copper content and show a significant pyramiding effect.Based on bioinformatics and haplotype analysis,TraesCS1B03G1265400 and TraesCS4A03G0093900 were proposed as candidate genes for regulating grain copper content.These results clarify the genetic architecture of grain copper content in wheat and provide a valuable foundation for developing germ-plasm with elevated grain copper content for wheat quality improvement breeding.
习千辉;徐梓瑗;刘梦梦;王宏艺;郎凯琳;井震海;陈锋;赵磊
河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046河南农业大学农学院/小麦玉米两熟高效生产全国重点实验室/河南粮食作物协同创新中心,河南 郑州 450046
小麦籽粒铜含量全基因组关联分析候选基因分析品质改良
wheatgrain copper contentgenome-wide association studycandidate gene analysisquality improvement
《作物学报》 2026 (6)
1604-1617,14
本研究由河南省科技研发计划联合基金项目(242301420132)和河南省高等学校重点科研项目(25A210020)资助.This study was supported by the Henan Provincial Science and Technology Research and Development Program Joint Fund(242301420132)and the Key Scientific Research Projects of Higher Education Institutions in Henan Province(25A210020).
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